Molecular characterization and expression analysis of TRIF, TRAF6, and TBK1 of golden pompano (Trachinotus ovatus)
Molecular characterization and expression analysis of TRIF, TRAF6, and TBK1 of golden pompano (Trachinotus ovatus)
复制标题
金鲳鱼 (Trachinotus ovatus) TRIF、TRAF6 和 TBK1 的分子特征和表达分析
DOI：
10.1016/j.fsi.2022.07.001
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发表时间：
2022
影响因子：
4.7
通讯作者：
Zhitao Qi
中科院分区：
文献类型：
--
作者：
Jinquan He;Shu Hu;Yushuai Xie;Youchuan Wei;Qihuan Zhang;Xiangyu Pi;Zhitao Qi
Toll/IL-1R domain-containing adaptor-inducing IFN-β (TRIF), tumor necrosis factor receptor-associated factor 6 (TRAF6) and TANK-binding kinase 1 (TBK1) are critical signal transducers in toll-like receptors (TLRs) signaling pathway. In the present study, TRIF, TRAF6 and TBK1 were characterized from golden pompano ( Trachinotus ovatus ), named as TroTRIF, TroTRAF6 and TroTBK1, respectively. The full cDNA length of TroTRIF, TroTRAF6 and TroTBK1 was 2297 bp, 2293 bp, and 2482 bp, which respectively encoded 589, 573 and 723 amino acids. The deduced amino acids sequences of TroTRIF, TroTRAF6 and TroTBK1 contained conserved motifs, similar to their counterparts in other vertebrates. Phylogenetic tree analysis revealed that TroTRIF, TroTRAF6 and TroTBK1 were well clustered with their counterparts in other fish species. Quantitative Real-Time PCR (qPCR) analysis showed that TroTRIF, TroTBK1 and TroTRAF6 were detected in all examined tissues of healthy fish, but shared distinct transcript levels. Moreover, the expressions of TroTRIF, TroTBK1 and TroTRAF6 were generally induced by polyriboinosinic-polyribocytidylic acid (polyI:C), lipopolysaccharide (LPS), and Vibrio alginolyticus stimulation in vivo , indicating their critical roles in the immune defense of golden pompano against pathogen invasion. Our results provide valuable information for understanding the functions of these genes in golden pompano. • TRIF, TRAF6 and TBK1 genes were cloned from golden pompano. • Sequence features of TRIF, TRAF6 and TBK1 were analyzed. • Expressional patterns of TRIF, TRAF6 and TBK1 in normal tissues were analyzed. • Expressions of TRIF, TRAF6 and TBK in spleen and HK following LPS, polyI:C, and V. alginolyticus challenge were analyzed.
