Finite element modeling of 3D human mesenchymal stem cell-seeded collagen matrices exposed to tensile strain.
Finite element modeling of 3D human mesenchymal stem cell-seeded collagen matrices exposed to tensile strain.
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DOI：
10.1016/j.jbiomech.2008.04.007
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发表时间：
2008-07
影响因子：
2.4
通讯作者：
T. W. Pfeiler;R. Sumanasinghe;E. Loboa
中科院分区：
文献类型：
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作者：
T. W. Pfeiler;R. Sumanasinghe;E. Loboa
人间充质干细胞（hMSCs）在组织工程中的应用是有吸引力的，因为它们具有广泛的自我复制和分化成多种细胞系的能力。实验表明，间充质干细胞受化学和机械信号的影响。然而，化学和机械相结合的体外培养条件，导致功能组织需要更多的了解。在本研究中，建立了有限元模型来评估在循环拉伸应变下，骨髓来源的hMSCs植入三维胶原基质中的局部加载条件。模型中使用的力学性能和几何数据是从我们实验室先前的研究和力学测试中通过实验获得的。建立8个有限元模型，模拟不同水平的循环拉伸应变（10%和12%）、培养基（完全生长和成骨分化）和培养时间（7和14天）下的三维hmsc种子胶原基质。通过有限元分析，确定全局施加10%和12%的单轴拉伸应变，导致局部应变分别高达18.3%和21.8%。我们还将模型结果与实验研究进行了比较，试图解释hMSC对10%和12%循环拉伸应变的响应差异。
The use of human mesenchymal stem cells (hMSCs) in tissue engineering is attractive due to their ability to extensively self-replicate and differentiate into a multitude of cell lineages. It has been experimentally established that hMSCs are influenced by chemical and mechanical signals. However, the combined chemical and mechanical in vitro culture conditions that lead to functional tissue require greater understanding. In this study, finite element models were created to evaluate the local loading conditions on bone marrow-derived hMSCs seeded in three-dimensional collagen matrices exposed to cyclic tensile strain. Mechanical property and geometry data used in the models were obtained experimentally from a previous study in our laboratory and from mechanical testing. Eight finite element models were created to simulate three-dimensional hMSC-seeded collagen matrices exposed to different levels of cyclic tensile strain (10% and 12%), culture media (complete growth and osteogenic differentiating), and durations of culture (7 and 14 days). Through finite element analysis, it was determined that globally applied uniaxial tensile strains of 10% and 12% resulted in local strains up to 18.3% and 21.8%, respectively. Model results were also compared to experimental studies in an attempt to explain observed differences between hMSC response to 10% and 12% cyclic tensile strain.