Effects of changes in dietary fatty acids on isolated skeletal muscle functions in rats.
Effects of changes in dietary fatty acids on isolated skeletal muscle functions in rats.
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膳食脂肪酸变化对大鼠离体骨骼肌功能的影响。
DOI：
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发表时间：
1996
影响因子：
3.3
通讯作者：
A. J. Hulbert
中科院分区：
文献类型：
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作者：
K. Ayre;A. J. Hulbert
研究了操纵膳食必需多不饱和脂肪酸水平对雄性Wistar大鼠离体骨骼肌功能的影响。使用三种等能饮食：必需脂肪酸缺乏饮食（EFAD）、必需（n-6）脂肪酸高的饮食[高（n-6）]和富含必需（n-3）脂肪酸的饮食[高（n-3）]。9周后，各组大鼠在每一个测试饮食喂养的股票饮食的实验室饲料为进一步的6周。肌肉功能进行了检查，使用电池的五个测试比目鱼肌（慢颤）和趾长伸肌（EDL;快颤）。测试包括单次肌肉抽搐、持续强直收缩、强直后增强、持续高频刺激和间歇性低频刺激。高剂量组（n-6）和高剂量组（n-3）的肌肉结果非常相似。然而，与高（n-6）和高（n-3）饮食相比，EFAD饮食导致肌肉张力显著降低，反应时间缩短。EFAD组比目鱼肌的峰值抽搐张力分别比高（n-6）和高（n-3）组低16-21%[方差分析（ANOVA），P < 0.01]。在高频刺激期间，来自EFAD大鼠的EDL肌肉疲劳快32%（ANOVA，P < 0.01）]。此外，EFAD组的抽搐收缩和半松弛时间显著减少5-7%（ANOVA，P < 0.01）。在间歇性低频刺激期间，EFAD组的比目鱼肌产生的张力比其他组低25-28%（ANOVA，P < 0.01），但EFAD组的EDL肌肉的耐力比高（n-6）组高20%（ANOVA，P < 0.05）。6周后的股票饮食，饮食组之间不再有任何差异。膳食脂肪酸的操纵导致显著的，但可逆的，在肌肉中的EFAD饮食喂养的大鼠的影响。
The effects of manipulating dietary levels of essential polyunsaturated fatty acids on the function of isolated skeletal muscles in male Wistar rats were examined. Three isoenergetic diets were used: an essential fatty acid-deficient diet (EFAD), a diet high in essential (n-6) fatty acids [High (n-6)], and a diet enriched with essential (n-3) fatty acids [High (n-3)]. After 9 wk, groups of rats on each test diet were fed a stock diet of laboratory chow for a further 6 wk. Muscle function was examined by using a battery of five tests for soleus (slow twitch) and extensor digitorum longus (EDL; fast twitch). Tests included single muscle twitches, sustained tetanic contractions, posttetanic potentiation, sustained high-frequency stimulation, and intermittent low-frequency stimulation. Results for muscles from the High (n-6) and High (n-3) groups were very similar. However, the EFAD diet resulted in significantly lower muscular tensions and reduced response times compared with the High (n-6) and High (n-3) diets. Peak twitch tension in soleus muscles was 16-21% less in the EFAD group than in the High (n-6) and High (n-3) groups, respectively [analysis of variance (ANOVA), P < 0.01). During high-frequency stimulation, EDL muscles from the EFAD rats fatigued 32% more quickly (ANOVA, P < 0.01)]. Also, twitch contraction and half-relaxation times were significantly 5-7% reduced in the EFAD group (ANOVA, P < 0.01). During intermittent low-frequency stimulation, soleus muscles from the EFAD group generated 25-28% less tension than did the other groups (ANOVA, P < 0.01), but in EDL muscles from the EFAD group, endurance was 20% greater than in the High (n-6) group (ANOVA, P < 0.05). After 6 wk on the stock diet, there were no longer any differences between the dietary groups. Manipulation of dietary fatty acids results in significant, but reversible, effects in muscles of rats fed an EFAD diet.