Latent infection of low-virulence anaerobic bacteria in degenerated lumbar intervertebral discs
Latent infection of low-virulence anaerobic bacteria in degenerated lumbar intervertebral discs
复制标题
退变腰椎间盘低毒力厌氧菌的潜伏感染
DOI：
10.1186/s12891-018-2373-3
复制
发表时间：
2018-12-20
影响因子：
2.3
通讯作者：
Chen, Yong
中科院分区：
文献类型：
--
作者：
Tang, Guoqing;Wang, Zhuo;Chen, Yong
背景退变椎间盘(IVDS)中是否存在潜在的低毒力厌氧菌仍存在争议。方法对80例腰椎间盘突出症患者行腰椎间盘摘除手术，检测退行性椎管内畸形中低毒力厌氧菌的流行情况，并分析细菌感染与临床症状的相关性。在确保无菌条件的严格方案下，术中取出80个椎间盘样本并进行微生物培养。同时，采集周围肌肉和韧带组织标本，作为污染标志物进行培养。结果80个培养盘中54个无菌，26个有细菌存在：痤疮丙酸杆菌21例，凝固酶阴性葡萄球菌5例。核磁共振显示细菌的存在与MC显著相关(P<；0.001)。然而，细菌感染与IVD退变的严重程度无显著相关性(P= 0.162)。结论我们的发现进一步证实了退行性IVD和P。痤疮是最常见的细菌。此外，IVDS中细菌的潜伏感染与MODIC改变有关。因此，低毒力厌氧菌可能在MC和腰椎间盘突出症的病理生理机制中起重要作用。
BackgroundThe existence of latent low-virulence anaerobic bacteria in degenerated intervertebral discs (IVDs) remains controversial. In this study, the prevalence of low-virulence anaerobic bacteria in degenerated IVDs was examined, and the correlation between bacterial infection and clinical symptoms was analysed.MethodsEighty patients with disc herniation who underwent discectomy were included in this study. Under a stringent protocol to ensure sterile conditions, 80 disc samples were intraoperatively retrieved and subjected to microbiological culture. Meanwhile, tissue samples from the surrounding muscle and ligaments were harvested and cultured as contamination markers. The severity of IVD degeneration and the prevalence of Modic changes (MCs) were assessed according to preoperative MRI analysis.ResultsOf the 80 cultured discs, 54 were sterile, and 26 showed the presence of bacteria:Propionibacterium acnes(21 cases) and coagulase-negativestaphylococci(5 cases). MRI revealed that the presence of bacteria was significantly associated with MCs (P<0.001). However, there was no significant association between bacterial infection and the severity of IVD degeneration (P= 0.162).ConclusionsOur findings further validated the presence of low-virulence anaerobic bacteria in degenerated IVDs, andP. acneswas the most frequent bacterium. In addition, the latent infection of bacteria in IVDs was associated with Modic changes. Therefore, low-virulence anaerobic bacteria may play a crucial role in the pathophysiology of MCs and lumbar disc herniation.