Two interdependent TRPV channel subunits, inactive and Nanchung, mediate hearing in Drosophila
Two interdependent TRPV channel subunits, inactive and Nanchung, mediate hearing in Drosophila
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DOI：
10.1523/jneurosci.1645-04.2004
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发表时间：
2004-10-13
影响因子：
5.3
通讯作者：
Kim, CS
中科院分区：
文献类型：
--
作者：
Gong, ZF;Son, WS;Kim, CS
果蝇的听觉依赖于触角弦音器官（约翰斯顿器官）中纤毛感觉神经元将触角振动转换为受体电位。我们先前发现，香草素受体亚家族（TRPV）通道亚基中的果蝇蛋白质Nanchung（NAN）定位于弦音纤毛，并且是产生声音诱发电位所需的（Kim等人，2003年）。在这里，我们表明，唯一的其他果蝇TRPV蛋白是突变的行为突变体失活（iav）。IAV蛋白在培养细胞中表达时形成低渗激活通道;在苍蝇中，它特异性地表达于弦音神经元中，定位于它们的纤毛并且是听觉所需的。IAV和NAN在缺乏其他蛋白的突变体的纤毛中均检测不到，表明它们都有助于体内的异源多聚体转导通道。功能性绿色荧光蛋白- IAV融合蛋白显示通道限于近端纤毛，限制了通道激活的模型。
Hearing in Drosophila depends on the transduction of antennal vibration into receptor potentials by ciliated sensory neurons in Johnston's organ, the antennal chordotonal organ. We previously found that a Drosophila protein in the vanilloid receptor subfamily (TRPV) channel subunit, Nanchung (NAN), is localized to the chordotonal cilia and required to generate sound-evoked potentials (Kim et al., 2003). Here, we show that the only other Drosophila TRPV protein is mutated in the behavioral mutant inactive (iav). The IAV protein forms a hypotonically activated channel when expressed in cultured cells; in flies, it is specifically expressed in the chordotonal neurons, localized to their cilia and required for hearing. IAV and NAN are each undetectable in cilia of mutants lacking the other protein, indicating that they both contribute to a heteromultimeric transduction channel in vivo. A functional green fluorescence protein - IAV fusion protein shows that the channel is restricted to the proximal cilium, constraining models for channel activation.