Synthesis of thermo- and acid-stable novel oligosaccharides by using dextransucrase with high concentration of sucrose
Synthesis of thermo- and acid-stable novel oligosaccharides by using dextransucrase with high concentration of sucrose
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DOI：
10.5458/jag.54.147
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发表时间：
2007-04
影响因子：
1.1
通讯作者：
S. Nam;Eunhye Ko;Xing-Ji Jin;V. Breton;E. Abada;Young-Min Kim;A. Kimura;Doman Kim
中科院分区：
文献类型：
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作者：
S. Nam;Eunhye Ko;Xing-Ji Jin;V. Breton;E. Abada;Young-Min Kim;A. Kimura;Doman Kim
本文提出了一种以蔗糖（2.5-4 M）为原料，利用从肠系膜Leuconostoc mesenteroides B-512FMCM中制备的葡聚糖（dextran蔗糖酶）合成热、酸稳定的低聚糖（TASOG）的方法。合成的低聚糖的聚合度（DP）为2 ~ 11。在140℃和pH 6.0条件下，TASOG的糖苷键在1小时内不被水解。在120℃条件下，TASOG在pH 2 ~ 4范围内保持稳定。本文还研究了以肠系膜乳杆菌B-1355C为原料，利用左旋蔗糖酶合成高浓度蔗糖（1 ~ 3 M）低聚果糖（TASOF）的方法。本方法合成的低聚糖的DP值为2 ~ 15以上。在120℃下，TASOF在ph值2 ~ 4范围内也保持稳定。反应产物中TASOF的比例为95.7%（不含单糖，4.3%为levan）。两种低聚糖均能有效抑制不溶性葡聚糖的形成，并抑制sobrinus链球菌的生长和产酸。TASOG和TASOF可以作为食品和饮料的甜味剂，在需要热稳定性和酸稳定性的地方，也可以作为潜在的龋齿抑制剂。
A method is presented for synthesizing thermo-, acid-stable glucooligosaccharides (TASOG) from sucrose (2.5-4 M) using a dextransucrase prepared from Leuconostoc mesenteroides B-512FMCM. The degree of polymerization (DP) of oligosaccharides synthesized was from 2 to 11. TASOG resisted hydrolysis of its glycosidic linkages at 140°C and pH 6.0 for 1 h. It was stable at pHs ranging from 2 to 4 at 120°C. A method for synthesizing fructo-oligosaccharides (TASOF) with high concentrations of sucrose (1-3 M) by using levansucrase prepared from L. mesenteroides B-1355C was also developed. The DP of oligosaccharides synthesized according to the present method ranged from 2 to over 15. The TASOF was also stable at pHs ranging from 2 to 4 under 120°C. The percentage of TASOF in the reaction digest was 95.7% (excluding monosaccharides; 4.3% was levan). Both oligosaccharides effectively inhibited the formation of insoluble glucan, and the growth and acid production of Streptococcus sobrinus. TASOG and TASOF potentially can be used as sweeteners for food and beverages where thermo- and acid-stable properties are required and as potential inhibitors of dental caries.