Enhanced reproductive toxicities induced by phthalates contaminated microplastics in male mice (Mus musculus)
Enhanced reproductive toxicities induced by phthalates contaminated microplastics in male mice (Mus musculus)
复制标题
邻苯二甲酸盐污染的微塑料导致雄性小鼠（Mus musculus）的生殖毒性增强
DOI：
10.1016/j.jhazmat.2020.124644
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发表时间：
2021-03-15
影响因子：
13.6
通讯作者：
Zhang, Yan
中科院分区：
文献类型：
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作者：
Deng, Yongfeng;Yan, Zehua;Zhang, Yan
研究表明，微塑料（MP）可以将邻苯二甲酸酯（PAEs）转运到小鼠的组织中。然而，MP对PAEs积累的影响及其联合毒性还有待深入研究。在这项研究中，邻苯二甲酸酯的生物累积和生殖毒性由于污染的MP暴露进行了调查。在暴露于PAE污染的MP 30天后，在肝脏和肠道中观察到PAE显著增加的积累，但在睾丸中没有观察到，这归因于MP在组织中的分布。在此，大多数微米大小的MP积累在肠道和肝脏，而只有少数纳米大小的MP进入支持细胞。与未经处理的MP和单独的PAEs相比，PAEs污染的MP诱导增强的生殖毒性表现为精子生理和精子发生的更大改变。增强的毒性也被证实的睾丸转录组学的改变和加剧的氧化应激诱导的PAE污染的MP。这些加重的生殖毒性不仅仅是由PAE引起的，也可能是由MP诱导的氧化应激的致敏作用引起的。我们的研究结果强调了由于MP和塑料添加剂的共同暴露对雄性陆生哺乳动物的潜在生殖毒性，并为MP和其他污染物的联合毒性机制提供了有价值的见解。
It has been demonstrated that microplastics (MPs) can transport phthalate esters (PAEs) into the tissues of mice. However, the influence of MPs on accumulation of PAEs and the combined toxicity need profound investigation. In this study, the bioaccumulation of PAEs and reproductive toxicity due to contaminated MPs exposure were investigated. After exposure to PAE-contaminated MPs for 30 days, significantly increased accumulation of PAE was observed in the liver and gut but not in the testis, which are ascribed to the distribution of MPs in tissues. Herein, most micro-size MPs accumulated in the gut and liver, while only a few nano-size MPs entered the Sertoli cells. Compared with virgin MPs and PAEs alone, PAE-contaminated MPs induced enhanced reproductive toxicities manifested by greater alterations in sperm physiology and spermatogenesis. The enhanced toxicities were also confirmed by the testicular transcriptomic alterations and aggravated oxidative stress induced by PAE-contaminated MPs. These aggravated reproductive toxicities were not caused solely by PAE, but may also be caused by the sensitization effect of oxidative stress induced by MPs. Our results highlight the potential reproductive toxicity on male terrestrial mammals due to co-exposure of MPs and plastic additives and provide valuable insights into the mechanism of combined toxicity of MPs and other pollutants.