Distribution patterns of soil microbial eukaryotes suggests widespread algivory by phagotrophic protists as an alternative pathway for nutrient cycling
Distribution patterns of soil microbial eukaryotes suggests widespread algivory by phagotrophic protists as an alternative pathway for nutrient cycling
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DOI：
10.1016/j.soilbio.2017.05.002
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发表时间：
2017-09-01
影响因子：
9.7
通讯作者：
Lara, Enrique
中科院分区：
文献类型：
--
作者：
Seppey, Christophe V. W.;Singer, David;Lara, Enrique
土壤环境DNA（eDNA）的高通量测序（HTS）可以评估土壤微真核生物的全部多样性。所得到的操作分类单位（OTU）可以分配到潜在的分类和功能的身份使用越来越完整的参考数据库。土壤eDNA的HTS揭示了潜在的真核生物原生生物的高度多样性和丰富性，从而挑战了土壤食菌原生生物（即微生物环）的主要食菌功能的范式。使用Illumina测序的土壤eDNA和针对SSU rRNA基因的V9区域，我们调查了分类和功能的多样性，分布和共存模式的土壤微型真核生物在三个土地利用类型：森林，草地和农田位于瑞士。每个CHU被分配到一个广泛的功能类别（光养生物，吞噬生物，反硝化生物，或寄生虫）。总OTU丰富度在三个土地利用类别是相似的，但社区组成显着不同的森林和其他土地利用。森林中真菌序列（特别是担子菌门）的比例最高，而光养生物（即土壤微藻）序列最少。7个OTU代表食食性原生生物，共占所有食食性序列的25%以上，与光养生物序列的总数显著相关，因此表明藻类。这些OTU中至少有三个对应于已知的藻类捕食者。这些结果表明，除了植物，土壤微藻代表了一个功能上重要的，但很少考虑到土壤中的碳输入，应该考虑到土壤养分循环建模时。(C)2017爱思唯尔有限公司版权所有
High-throughput sequencing (HTS) of soil environmental DNA (eDNA) allows assessing the full diversity of soil micro-eukaryotes. The resulting operational taxonomic units (OTUs) can be assigned to potential taxonomic and functional identities using increasingly complete reference databases. HTS of soil eDNA is revealing a high diversity and abundance of potential eukaryovorous protists, thus challenging the paradigm of the predominantly bacterivorous function of soil phagotrophic protists (i.e. microbial loop). Using Illumina sequencing of soil eDNA and targeting the V9 region of the SSU rRNA gene, we investigated the taxonomic and functional diversities, distribution and co-occurrence patterns of soil micro-eukaryotes in three land-use categories: forests, meadows and croplands located in Switzerland. Each CHU was assigned to a broad functional category (phototrophs, phagotrophs, osmotrophs, or parasites).Total OTU richness was similar in the three land-use categories, but community composition differed significantly between forests and other land-uses. The proportion of fungal sequences (especially Basidiomycota) was highest, and phototroph (i.e. soil microalgae) sequences least abundant in forests. Seven OTUs representing phagotrophic protists, together accounting for >25% of all phagotroph sequences, were significantly correlated to the total number of phototroph sequences, thus suggesting algivory. At least three of these OTUs corresponded to known algal predators.These results suggest that beyond plants, soil microalgae represent a functionally significant but rarely considered input of carbon in soils that should be taken into account when modelling soil nutrient cycling. (C) 2017 Elsevier Ltd. All rights reserved.