Immunogenicity of Bovine and Leporine Articular Chondrocytes and Meniscus Cells
Immunogenicity of Bovine and Leporine Articular Chondrocytes and Meniscus Cells
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DOI：
10.1089/ten.tea.2011.0226
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发表时间：
2012-03-01
影响因子：
4.1
通讯作者：
Athanasiou, Kyriacos A.
中科院分区：
文献类型：
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作者：
Huey, Daniel J.;Sanchez-Adams, Johannah;Athanasiou, Kyriacos A.
免疫排斥是任何异体或异种移植的主要问题。对于软骨组织工程策略的体内研究，通常采用小动物模型，如leporine模型。许多研究报告在同种异体或异种植入天然关节和半月板软骨时很少或没有免疫排斥反应。本研究探讨了牛关节软骨细胞（ACs）和半月板细胞（MCs）是否具有免疫特权特性，因为它们能够在体外刺激外周血单个核细胞（PBMCs）的增殖。共培养6天后，没有一种类型的细胞在leporine pbmc中引起增殖反应，表明这些细胞在体内可能不会引起免疫排斥反应。主要组织相容性复合体类（MHC） I和II以及共刺激因子CD80和CD86的逆转录酶聚合酶链反应分析显示，所有细胞类型都产生MHC I和II的信使RNA，但只有部分细胞CD80或CD86阳性，没有细胞同时对两种共刺激因子都呈阳性。流式细胞术发现，牛MCs和ACs均显示MHC II (MCs: 32.5%, ACs: 14.4%)，而只有leporine ACs呈MHC II阳性（7.5%）。尽管在分离细胞中存在MHC I和II，但在完整的牛或麻黄透明软骨或半月板组织中未观察到MHC I和II。尽管存在一些MHC II和共刺激因子，但所研究的细胞类型都不能引起PBMC增殖。这些发现表明，牛和麻素MCs和ACs具有相似的免疫特权，支持它们作为工程软骨的异体和异种细胞来源。
Immune rejection is a major concern for any allogeneic or xenogeneic graft. For in vivo investigations of cartilage tissue engineering strategies, small animal models such as the leporine model are commonly employed. Many studies report little to no immune rejection upon allogeneic or xenogeneic implantation of native articular and meniscal cartilages. This study investigated whether bovine and leporine articular chondrocytes (ACs) and meniscus cells (MCs) have immunoprivileged characteristics because of their ability to stimulate proliferation of leporine peripheral blood mononuclear cells (PBMCs) in vitro. After 6 days of co-culture, none of the cell types caused a proliferative response in the leporine PBMCs, indicating that these cells may not elicit immune rejection in vivo. Reverse transcriptase polymerase chain reaction analysis for major histocompatibility complex class (MHC) I and II and costimulation factors CD80 and CD86 revealed that all cell types produced messenger RNA for MHC I and II, but only some were CD80 or CD86 positive, and none were positive for both costimulation factors. Flow cytometry found that bovine MCs and ACs displayed MHC II (MCs: 32.5%, ACs: 14.4%), whereas only leporine ACs were MHC II positive (7.5%). Although present in isolated cells, MHC I and II were not observed in intact bovine or leporine hyaline cartilage or meniscus tissues. Despite some presence of MHC II and costimulation factors, none of the cell types studied were able to cause PBMC proliferation. These findings indicate that bovine and leporine MCs and ACs share a similar immunoprivileged profile, bolstering their use as allogeneic and xenogeneic cell sources for engineered cartilage.