Immobilization of avidin on the functionalized carbon nanotubes
Immobilization of avidin on the functionalized carbon nanotubes
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DOI：
10.1016/j.synthmet.2006.06.003
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发表时间：
2006-07-01
期刊：
影响因子：
4.4
通讯作者：
Shin, Hyung-Shik
中科院分区：
文献类型：
--
作者：
Kim, Young-Soon;Cho, Joong-Hee;Shin, Hyung-Shik
通过功能化多壁碳纳米管（MWCNTs）固定化抗生物素蛋白。用硝酸和硫酸混合物（1：3，优化比例）处理导致从傅里叶变换红外吸收光谱（FTIR）测量观察到的纳米管的官能化。将亲和素与N-羟基琥珀酰亚胺（NHS）和1-乙基-3-（3-二甲氨基丙基）-碳二亚胺盐酸盐（EDC）的溶液偶联，然后固定在纳米管上。X射线光电子能谱（XPS）研究显示C Is的峰位置向低能侧移动，并且谱带随着处理/固定而变化。光电子峰位的肩部和位移表明表面层石墨结构的破坏。SEM图像显示，功能化后，纳米管被视为具有开放的端部，颗粒状的表面，并连接在一起。这表明，在处理后，碳纳米管的反应性增加，在端部以及在侧壁。据信NHS通常在EDC存在下辅助碳二亚胺偶联，与胺官能团反应以产生酰胺键。碳二亚胺通过活化羧基来催化羧酸和胺之间的酰胺键的形成。FTIR和XPS分析表明，亲和素复合物与功能化碳纳米管形成共价键。(c)2006 Elsevier B.V保留所有权利。
Immobilization of avidin was carried out by functionalizing the multi-wall carbon nanotubes (MWCNTs). Treatment with nitric acid and sulfuric acid mixture (1:3, optimized ratio) leads to the functionalization of nanotubes as observed from Fourier transform infrared absorption spectroscopy (FTIR) measurements. Avidin was coupled with the solution of N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylamino propyl)-carbodiimide hydrochloride (EDC) and then immobilized on nanotubes. X-ray photoelectron spectroscopic (XPS) studies show a shift in the peak position of C Is towards lower energy side and changes in the bands with treatment/immobilization. The shoulders and shift in photoelectron peak positions indicate the destruction of the graphite structure of the surface layer. SEM images showed that after functionalization, the nanotubes are seen with open ends, granular surface and are joined together. This indicates that after treatment, the CNTs reactivity increased at the ends as well as at the sidewalls. It is believed that the NHS often assist the carbodiimide coupling in the presence of EDC, reacts with the amine function to yield the amide bond. The carbodiimide catalyzes the formation of amide bonds between carboxylic acids and amines by activating carboxyl. The reaction of complex containing avidin can form a covalent bond with functionalized carbon nanotubes as observed from FTIR and XPS measurements. (c) 2006 Elsevier B.V All rights reserved.