The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin
The opportunistic marine pathogen Vibrio parahaemolyticus becomes virulent by acquiring a plasmid that expresses a deadly toxin
复制标题
DOI：
10.1073/pnas.1503129112
复制
发表时间：
2015-08-25
影响因子：
11.1
通讯作者：
Lo, Chu-Fang
中科院分区：
文献类型：
--
作者：
Lee, Chung-Te;Chen, I-Tung;Lo, Chu-Fang
急性肝胰腺坏死病（AHPND）是由副溶血性弧菌引起的一种严重的、新出现的对虾疾病，已经给养殖对虾业造成了巨大的损失。到目前为止，其致病机制仍不清楚。在这里，我们发现一株引起ahpnd的副溶血性弧菌含有一个70 kbp的质粒（pVA1），该质粒具有分离后杀伤系统，并且通过自然缺失或实验缺失光habduus昆虫相关（Pir）毒素PirA和PirB的质粒编码同源物，从而消除了引起疾病的能力。我们测定了副溶血性弧菌PirA和PirB（PirA（vp）和PirB(vp)）蛋白的晶体结构，发现PirA(vp)/PirB（vp）的整体结构拓扑结构与芽孢杆菌Cry杀虫毒素样蛋白非常相似，尽管序列同源性较低(
Acute hepatopancreatic necrosis disease (AHPND) is a severe, newly emergent penaeid shrimp disease caused by Vibrio parahaemolyticus that has already led to tremendous losses in the cultured shrimp industry. Until now, its disease-causing mechanism has remained unclear. Here we show that an AHPND-causing strain of V. parahaemolyticus contains a 70-kbp plasmid (pVA1) with a postsegregational killing system, and that the ability to cause disease is abolished by the natural absence or experimental deletion of the plasmid-encoded homologs of the Photorhabdus insect-related (Pir) toxins PirA and PirB. We determined the crystal structure of the V. parahaemolyticus PirA and PirB (PirA(vp) and PirB(vp)) proteins and found that the overall structural topology of PirA(vp)/PirB(vp) is very similar to that of the Bacillus Cry insecticidal toxin-like proteins, despite the low sequence identity (