Presence and characterization of methicillin-resistant Staphylococcus aureus co-carrying the multidrug resistance genes cfr and lsa(E) in retail food in China
Presence and characterization of methicillin-resistant Staphylococcus aureus co-carrying the multidrug resistance genes cfr and lsa(E) in retail food in China
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中国零售食品中同时携带多药耐药基因 cfr 和 lsa(E) 的耐甲氧西林金黄色葡萄球菌的存在及其特征
DOI：
10.1016/j.ijfoodmicro.2021.109512
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发表时间：
2022
影响因子：
5.4
通讯作者：
Zhang Y.
中科院分区：
文献类型：
--
作者：
Zhang F.;Wu S.;Lei T.;Wu Q.;Zhang J.;Huang J.;Dai J.;Chen M.;Ding Y.;Wang J.;Wei X.;Zhang Y.
金黄色葡萄球菌是一种与细菌中毒相关的重要致病菌，由于其多重耐药而难以救治。Frecandlsa(E)基因既可引起多重耐药，也可分别在多种葡萄球菌中发现。在本研究中，我们发现了一种耐甲氧西林的细菌。金黄色葡萄球菌(MRSA)2868B2株于2015年从杭州的一个冷冻饺子样品中分离出来，共携带这两种不同的多药耐药基因。进一步分析表明，该菌株对18种以上抗生素耐药，对氟苯尼考、氯霉素、克林霉素、替阿莫林、红霉素、氨苄西林、头孢吡肟、头孢他啶、卡那霉素、链霉素、四环素、甲氧嘧啶-磺胺甲恶唑和利奈唑胺表现出高水平耐药(MIC50=18.8μg/mL)。对这些抗性基因的遗传环境和其他基因组特征进行了全基因组测序。该基因位于单质粒p2868B2(39,159个碱基)上，与以前在人和动物中发现的许多质粒有相当大的相似性。P2868B2含有插入序列(Is)元件IS21-558，它允许cfr插入到Tn558中，并在cfr的迁移率中发挥重要作用。此外，还发现了一个新的多药耐药区(36.9kb)，该区域含有9个新的抗生素耐药基因(Args)(aadD、aadE、aacA-apHD、spc、Lnu(B)、lsa(E)、etL、ermCandblaZ)。多药耐药区含有4个具有活性的IS257拷贝，可介导含有Args和ISS的4个环状结构的形成。此外，在MRSA 2868B2基因组中还发现了编码多种毒力因子和影响多种细胞黏附特性的基因。本研究证实了一株MRSA菌株中同时存在Frandlsa(E)基因，多个ARG簇中的质粒和IS257的存在可以促进ARG的转移和扩散。此外，食品相关病原菌中存在如此多的ARGs和毒力基因，可能严重危及临床治疗的有效性，威胁公众健康，应引起公众的关注，这些基因在食品相关样品中的可追溯性需要进一步监测。
Staphylococcus aureus is an important food-related pathogen associated with bacterial poisoning that is difficult to treat due to its multidrug resistance. Thecfrandlsa(E) genes both cause multiple drug resistance and have been identified in numerousStaphylococcusspecies, respectively. In this study, we found that a methicillin-resistantS. aureus(MRSA) strain, 2868B2, which was isolated from a sample of frozen dumplings in Hangzhou in 2015, co-carried these two different multidrug resistance genes. Further analysis showed that this strain was resistant to more than 18 antibiotics and expressed high-level resistance to florfenicol, chloramphenicol, clindamycin, tiamulin, erythromycin, ampicillin, cefepime, ceftazidime, kanamycin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole and linezolid (MIC = 8 μg/mL). Whole genome sequencing was performed to characterize the genetic environment of these resistance genes and other genomic features. Thecfrgene was located on the single plasmid p2868B2 (39,159 bp), which demonstrated considerable similarity to many plasmids previously identified in humans and animals. p2868B2 contained the insertion sequence (IS) element IS21–558, which allowed the insertion ofcfrinto Tn558and played an important role in the mobility ofcfr. Additionally, a novel multidrug resistance region (36.9 kb) harbouringlsa(E) along with nine additional antibiotic resistance genes (ARGs) (aadD,aadE,aacA-aphD,spc,lnu(B),lsa(E),tetL,ermCandblaZ) was identified. The multidrug resistance region harboured four copies of IS257that were active and can mediate the formation of four circular structures containing ARGs and ISs. In addition, genes encoding various virulence factors and affecting multiple cell adhesion properties were identified in the genome of MRSA 2868B2. This study confirmed that thecfrandlsa(E) genes coexist in one MRSA strain and the presence of plasmid and IS257in the multi-ARG cluster can promote both ARG transfer and dissemination. Furthermore, the presence of so many ARGs and virulence genes in food-related pathogens may seriously compromise the effectiveness of clinical therapy and threaten public health, its occurrence should pay public attention and the traceability of these genes in food-related samples needs further surveillance.