Cyp26C1 encodes a novel retinoic acid-metabolizing enzyme expressed in the hindbrain, inner ear, first branchial arch and tooth buds during murine development
Cyp26C1 encodes a novel retinoic acid-metabolizing enzyme expressed in the hindbrain, inner ear, first branchial arch and tooth buds during murine development
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DOI：
10.1016/s1567-133x(03)00066-8
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发表时间：
2003-08-01
影响因子：
1.2
通讯作者：
Petkovich, M
中科院分区：
文献类型：
--
作者：
Tahayato, A;Dollé, P;Petkovich, M
视黄酸 (RA) 是维生素 A 的活性代谢物，是参与胚胎发育过程中组织形态发生的重要信号分子。 RA 的分布和浓度在胚胎发生过程中通过 RA 合成 (RALDH) 和代谢 (CYP26) 酶的平衡互补活性进行精确调节。在这里，我们描述了属于 CYP26 家族的新型鼠 P450 细胞色素 mCYP26C1 的鉴定。序列比对表明，mCYP26C1 与 mCYP26B1 的相关性比 mCYP26A1 更密切。在早期发育阶段 (E8.0-E8.5)，rnCyp26C1 在预期菱形节 2 和 4、第一鳃弓以及沿着邻近后脑头侧的侧面间质中表达。在 E9.5，mCyp26C1 表达持续存在于菱形 2 以及第一鳃弓的上颌骨和下颌骨成分中，并且在侧颈间质中被强烈诱导。到妊娠中期，mCyp26C1 在颈部间质和第一鳃弓的上颌骨中微弱表达。在E11.5时，mCyp26C1仅可见于颈侧间质的窄带中。在妊娠晚期，rnCyp26C1 在内耳上皮中表现出区域特异性表达，并在发育中的牙齿的内牙上皮中表现出持续表达。这种表达模式表明 mCYP26C1 可能在胚胎发育过程中保护后脑、第一鳃弓、耳囊和牙芽免受 RA 暴露方面发挥重要作用。 (C) 2003 Elsevier Science B.V. 保留所有权利。
Retinoic acid (RA), an active metabolite of vitamin A, is a crucial signaling molecule involved in tissue morphogenesis during embryonic development. RA distribution and concentration is precisely regulated during embryogenesis by balanced complementary activities of RA synthesizing (RALDH) and metabolizing (CYP26) enzymes. Here, we describe the identification of a novel murine P450 cytochrome belonging to the CYP26 family, mCYP26C1. Sequence alignment show that mCYP26C1 is more closely related to mCYP26B1 than mCYP26A1. At early developmental stages (E8.0-E8.5), rnCyp26C1 is expressed in prospective rhombomeres 2 and 4, in the first branchial arch and along the lateral surface mesenchyme adjacent to the rostral hindbrain. At E9.5, mCyp26C1 expression persists in rhombomere 2 and in the maxillary and mandibular components of the first branchial arch, and is strongly induced in the lateral cervical mesenchyme. By mid-gestation, mCyp26C1 is weakly expressed in the cervical mesenchyme and in the maxillary component of the first branchial arch. At E11.5, mCyp26C1 can only be seen in a narrow band in the lateral cervical mesenchyme. During late gestation, rnCyp26C1 exhibits region-specific expression in the inner ear epithelium and a persistent expression in the inner dental epithelium of the developing teeth. This pattern of expression suggests that mCYP26C1 may play an important role in protecting the hindbrain, first branchial arch, otocyst and tooth buds against RA exposure during embryonic development. (C) 2003 Elsevier Science B.V. All rights reserved.