Effect of lumbar nerve root compression on primary sensory neurons and their central branches - Changes in the nociceptive neuropeptides substance P and somatostatin
Effect of lumbar nerve root compression on primary sensory neurons and their central branches - Changes in the nociceptive neuropeptides substance P and somatostatin
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DOI：
10.1097/01.brs.0000152377.72468.f4
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发表时间：
2005-02-01
期刊：
影响因子：
3
通讯作者：
Yoshizawa, H
中科院分区：
文献类型：
--
作者：
Kobayashi, S;Kokubo, Y;Yoshizawa, H
研究设计。本研究使用体内模型检查了腰神经根压迫对轴突流中伤害性神经肽的影响。 目的。目的是通过使用免疫组织化学技术检测 P 物质 (SP) 和生长抑素 (SOM) 来研究神经根受压后轴突血流的变化，这些物质被认为与温度和痛觉有关。背景数据摘要。机械压迫引起的根内血流紊乱和神经纤维变形被认为与以根性症状为特征的疾病（例如腰椎间盘突出症和腰椎管狭窄症）的病理生理学有关。然而，对于神经根受压引起的轴突血流变化的研究却很少。方法。在狗身上，使用四种不同压力的夹子压迫腰神经根。压迫24小时或1周后，采用免疫组化法检测脊髓背角、背根、背根神经节SP、SOM水平的变化。结果。压迫24小时后，背根的轴突流动受损，在压迫部位远端观察到SP和SOM的积累，并且对这些神经递质呈阳性反应的背根神经节细胞数量减少。压迫1周导致脊髓背角SP-和SOM-阳性纤维数量减少。结论。直接神经压迫导致的轴突血流变化可能会影响轴突内流动的神经递质的代谢，可能是神经功能下降的主要原因。
Study Design. This study examined the effect of lumbar nerve root compression on nociceptive neuropeptides in the axonal flow using an in vivo model.Objectives. The aim was to investigate changes in axonal flow after nerve root compression by using immunohistochemical techniques to detect substance P (SP) and somatostatin (SOM), which is thought to be involved in temperature and pain sensation.Summary of Background Data. Disturbance of intraradicular blood flow and nerve fiber deformation caused by mechanical compression are thought to be involved in the pathophysiology of diseases characterized by radicular symptoms, such as lumbar disc herniation and lumbar canal stenosis. However, little research has been conducted into the changes of axonal flow associated with nerve root compression.Methods. In dogs, the lumbar nerve roots were compressed using four types of clips with different pressures. Changes of SP and SOM levels in the spinal dorsal horn, dorsal root, and dorsal root ganglions were examined immunohistochemically after compression for 24 hours or 1 week.Results. After compression for 24 hours, axonal flow in the dorsal root was impaired, accumulation of SP and SOM was observed distal to the site of compression, and there was a decrease in the number of dorsal root ganglion cells showing positively for these neurotransmitters. Compression for 1 week resulted in a decrease in the number of SP- and SOM-positive fibers in the spinal dorsal horn.Conclusion. Change of axonal flow resulting from direct nerve compression could affect the metabolism of neurotransmitters that flow inside the axons and may be a primary cause of the decline in nerve function.