Screening and Identifying Antioxidative Components in Ginkgo biloba Pollen by DPPH-HPLC-PAD Coupled with HPLC-ESI-MS2.
Screening and Identifying Antioxidative Components in Ginkgo biloba Pollen by DPPH-HPLC-PAD Coupled with HPLC-ESI-MS2.
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DPPH-HPLC-PAD 联用 HPLC-ESI-MS 筛选鉴定银杏花粉中的抗氧化成分2
DOI：
10.1371/journal.pone.0170141
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Qiu J;Chen X;Netrusov AI;Zhou Q;Guo D;Liu X;He H;Xin X;Wang Y;Chen L
银杏（Ginkgo biloba）是一种存在于数十亿年前的古老树种，其叶和坚果在中国被用作草药和食品，而其花粉迄今为止除了授粉外没有任何应用。为了评价银杏花粉的抗氧化活性，快速筛选其抗氧化活性成分，测定了银杏花粉对1，1-二苯基-2-苦基肼（DPPH）的清除能力、总黄酮、总酚和原花青素，并与银杏叶和银杏果仁进行了比较。采用离线DPPH-HPLC-PAD和HPLC-ESI-MS 2对银杏花粉中抗氧化黄酮类化合物进行了筛选和鉴定。结果表明，银杏花粉清除DPPH自由基的能力远高于银杏果仁，但低于银杏叶，而银杏花粉中总黄酮含量约为银杏叶的4.37倍。进一步研究发现，银杏花粉中主要的黄酮醇苷元是山萘酚，占总苷元（包括槲皮素、山萘酚和异鼠李素）的96.71%，银杏花粉中主要的黄酮类成分是黄酮苷。最后，筛选出10个抗氧化峰，经鉴定为黄酮类化合物（包括山奈酚和9个黄酮苷），因此黄酮类化合物很可能是银杏叶中的主要抗氧化成分，其中3个新的山奈酚苷（峰1、2和3）为首次在银杏花粉中发现，这3个峰在银杏中从未发现过。
The Ginkgo biloba is one of ancient trees that exists from billions of years ago, its leaf and nut are used as herbs and foods in China, while so far its pollen does not have any application except pollination. In order to evaluate the antioxidant activity of Ginkgo biloba pollen, and rapidly screen its antioxidative components, the 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, total flavonoid, total phenol, and proanthocyanidin of Ginkgo biloba pollen were determined and compared with those of Ginkgo biloba leaf and nut, and the off-line DPPH-HPLC-PAD and HPLC-ESI-MS2 were applied for screening and identifying the antioxidant flavonoids in Ginkgo biloba pollen. The results showed that the DPPH scavenging ability of Ginkgo biloba pollen was much higher than Ginkgo biloba nut, but lower than Ginkgo biloba leaf, while the total content of flavonoid in Ginkgo biloba pollen was approximately 4.37 times higher than in Ginkgo biloba leaf. Further studies found that the major flavonol aglycone in Ginkgo biloba pollen was kaempferol, which accounted for 96.71% of the total aglycones (includes quercetin, kaempferol and isorhamnetin), and the main flavonoid components in Ginkgo biloba pollen were flavonoid glycosides. Finally, ten antioxidant peaks were screened and identified to be flavonoids (including kaempferol and nine flavonoid glycosides), so flavonoids were likely to be the main antioxidant components in GP, and among them, three novel kaempferol glycosides (peaks 1, 2, and 3) were found in Ginkgo biloba pollen for the first time, which had never been found in Ginkgo biloba.