Nanog expression in mouse germ cell development
Nanog expression in mouse germ cell development
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DOI：
10.1016/j.modgep.2005.03.001
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发表时间：
2005-06-01
影响因子：
1.2
通讯作者：
Tada, T
中科院分区：
文献类型：
--
作者：
Yamaguchi, S;Kimura, H;Tada, T
Nanog是新近发现的一种具有同源结构域的转录因子，表达于植入前和移植后早期胚胎的多潜能细胞以及胚胎干细胞和胚胎生殖细胞。基因敲除实验表明，Nanog在维持干细胞的多能性方面发挥了关键作用。重要的是，Nanog在E11.5和E12.5小鼠胚胎的原始生殖细胞(PGC)中高表达。然而，它在生殖细胞中的时空表达模式和功能在很大程度上是未知的。为了解决这些问题，用抗NANOG和抗Stella/PGC7抗体对整个胚胎和胚胎的冰冻切片进行了免疫染色。在E7.25-E7.5胚胎定植的PGCs中可检测到Nanog的表达，在E7.75-E8.0胚胎的迁移PGCs中可检测到Nanog的表达。在E9.5和E10.5胚胎中迁移以及在E1.5和E12.5胚胎生殖器脊定植的雄性和雌性PGCs均呈NANOG免疫阳性，但在发育后期性别间NANOG表达模式不同。在E13.5和E14.5胚胎的雌性性腺PGCs中，NANOG在突触复合体特异性蛋白SCP3阳性的生殖细胞中检测不到，而在E14.5-E16.5胚胎的雄性PGCs中，NANOG阳性生殖细胞的数量在有丝分裂停止期间急剧减少。成年小鼠的睾丸和卵巢中未检测到NANOG阳性生殖细胞。因此，在生殖细胞发育中，NANOG在增殖的生殖细胞中表达，在增殖的生殖细胞中，核重新编程正在进行。(C)2005 Elsevier B.V.保留所有权利。
Nanog is a newly identified transcriptional factor bearing a homeodomain and expressed in pluripotential cells of preimplantation and early postimplantation embryos, and embryonic stem (ES) and embryonic germ (EG) cells. Knockout experiments indicate that Nanog functions as a key player in maintaining the pluripotency of stem cells. Importantly, Nanog expression is highly expressed in primordial germ cells (PGCs) of E11.5 and E12.5 mouse embryos. However, its temporal and spatial expression pattern and function in germ cells are largely unknown. To address these issues, whole embryos and cryosections of embryos were immumostained with anti-NANOG and anti-STELLA/PGC7 antibodies. NANOG expression, repressed in colonized PGCs of E7.25-E7.5 embryos, became detectable in migrating PGCs of E7.75-E8.0 embryos. Both male and female PGCs migrating in E9.5 and E10.5 embryos and colonizing the genital ridges of El 1.5 and E12.5 embryos were positive for NANOG immumostaining, while the NANOG expression pattern differed between the sexes in the later developmental stage. In female gonadal PGCs of E13.5 and E14.5 embryos, NANOG became undetectable in germ cells positive for the synaptonemal complex-specific protein SCP3, while in male PGCs of E14.5-E16.5 embryos, the number of NANOG-positive germ cells drastically decreased during the mitotic arrest. No germ cells positive for NANOG were detectable in testes and ovaries of adult mice. Thus, in germ cell development, NANOG is expressed in proliferating germ cells, in which nuclear reprogramming is progressing. (c) 2005 Elsevier B.V. All rights reserved.