The effects of DGAT1 and DGAT2 mRNA expression on fat deposition in fatty and lean breeds of pig
The effects of DGAT1 and DGAT2 mRNA expression on fat deposition in fatty and lean breeds of pig
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DOI：
10.1016/j.livsci.2011.04.007
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发表时间：
2011-09-01
影响因子：
1.8
通讯作者：
Yang, L.
中科院分区：
文献类型：
--
作者：
Cui, J. X.;Zeng, Y. Q.;Yang, L.
二酰基甘油三酯酰基转移酶（DGAT）在动物脂肪沉积中起着关键的调节作用。肌内脂肪（IMF）含量和背膘厚度（BFT）是决定猪肉品质的重要因素。因此，本研究旨在通过实时荧光定量PCR技术，研究莱芜猪（LW, n = 12，肥猪）、鲁来黑猪（LL, n = 12）和大白猪（LY, n = 12，瘦猪）3个重要猪种DGAT mRNA表达与IMF含量、BFT之间的关系。36头猪采用去势公猪（114±2 kg），分别取背最长肌、背脂肪和肝脏。结果表明，大鼠大鼠DGAT mRNA表达量最大，大鼠大鼠最小。最后，DGAT1基因的表达与BFT呈正相关，与IMF无关；DGAT2基因与IMF呈显著正相关，与BFT无显著正相关，说明DGAT2基因在脂肪沉积中具有不同的功能；此外，不同品种间IMF含量差异极显著（P < 0.01），不同BFT间差异不显著（P < 0.05）。根据DGAT1和DGAT2的表达结果，我们认为IMF和BFT的功能并不相同，IMF和BFT的沉积受不同机制的调控。(C) 2011 Elsevier B.V.版权所有
Diacylgycerol acyltransferase (DGAT) plays a key modulatory role in animal fat deposition. Intramuscular fat (IMF) content and backfat thickness (BFT) were important factors in establishing pork quality. Therefore, the objectives of the current study were to elucidate the relationship between DGAT mRNA expression with IMF content, BFT by quantitative real-time PCR in three important pig breeds, including Laiwu pig (LW, n = 12, fatty), Lulai Black (LL, n = 12) and Large White (LY, n = 12, lean). A total of 36 pigs were castrated boars (114 +/- 2 kg) and taken from longissimus dorsi muscle, backfat and liver. Results showed that the mRNA level of DGAT was maximal in LW, the minimal in LY. Finally, the expression of DGAT1 gene was positively correlated with BFT and not IMF; DGAT2 gene was positively correlated with IMF and not BFT The results indicated that the two genes have a different function in fat deposition; in addition, the IMF contents were significantly different among breeds (P < 0.01), and not significantly different among BFT (P > 0.05). Based on results of DGAT1 and DGAT2 expression we suggested that the function of IMF and BFT was not the same and the deposition of IMF and BFT was regulated by different mechanisms. (C) 2011 Elsevier B.V. All rights reserved.