Hyaluronan enhances wound repair and increases collagen III in aged dermal wounds.
Hyaluronan enhances wound repair and increases collagen III in aged dermal wounds.
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DOI：
10.1111/wrr.12192
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发表时间：
2014-07
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影响因子：
--
通讯作者：
Reed MJ
中科院分区：
文献类型：
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作者：
Damodarasamy M;Johnson RS;Bentov I;MacCoss MJ;Vernon RB;Reed MJ
细胞外基质中的炎症相关变化有助于延迟老化中的伤口修复。Hybryonan是一种线性非硫酸化糖胺聚糖，在伤口愈合过程中促进关键细胞外基质组分（如间质胶原）的合成和组装。透明质酸的生物学效应部分由透明质酸大小介导。我们先前已经确定，相对于年轻小鼠，老年小鼠的皮肤伤口在产生较低分子量透明质酸（定义为<300 kDa）方面存在缺陷。在这里，我们测试了2，250或1,000 kDa大小的外源性透明质酸对老年小鼠全层切除伤口的影响。仅用250 kDa透明质酸（HA 250）处理的伤口比仅接受载体（水）的伤口显著改善。HA 250治疗与透明质酸受体CD 44和RHAMM以及胶原III和I的转录物表达增加相关。通过质谱法和蛋白质印迹法对真皮蛋白质含量的分析证实，相对于对照伤口，用HA 250处理的伤口中胶原蛋白III的表达显著增加。总之，我们发现HA 250改善了伤口修复，并增加了老化皮肤伤口中胶原蛋白III的合成。
Age-related changes in the extracellular matrix contribute to delayed wound repair in aging. Hyaluronan, a linear nonsulfated glycosaminoglycan, promotes synthesis and assembly of key extracellular matrix components, such as the interstitial collagens, during wound healing. The biological effects of hyaluronan are mediated, in part, by hyaluronan size. We have previously determined that dermal wounds in aged mice, relative to young mice, have deficits in the generation of lower molecular weight hyaluronan (defined as <300 kDa). Here, we tested the effect of exogenous hyaluronan of 2, 250, or 1,000 kDa sizes on full-thickness excisional wounds in aged mice. Only wounds treated with 250 kDa hyaluronan (HA250) were significantly improved over wounds that received carrier (water) alone. Treatment with HA250 was associated with increased expression of transcripts for the hyaluronan receptors CD44 and RHAMM, as well as collagens III and I. Analyses of dermal protein content by mass spectrometry and Western blotting confirmed significantly increased expression of collagen III in wounds treated with HA250 relative to control wounds. In summary, we find that HA250 improves wound repair and increases the synthesis of collagen III in aged dermal wounds.