Dose- and age-dependent effects of prolactin (PRL) on luteinizing hormone- and PRL-binding sites in rat leydig cell homogenates.
Dose- and age-dependent effects of prolactin (PRL) on luteinizing hormone- and PRL-binding sites in rat leydig cell homogenates.
复制标题
催乳素 (PRL) 对大鼠间质细胞匀浆中黄体生成素和 PRL 结合位点的剂量和年龄依赖性影响。
DOI：
10.1210/endo-107-5-1639
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发表时间：
1980
期刊：
影响因子：
4.8
通讯作者：
B. Saxena
中科院分区：
文献类型：
--
作者：
P. Morris;B. Saxena
将不同剂量的绵羊催乳素(oPRL；NIH-P-S12)分别注射于幼龄(22日龄)和成年(82日龄)SD大鼠体内2天，观察PRL对间质细胞匀浆中hCG和hPRL结合部位的影响。由于人绒毛膜促性腺激素和促黄体生成素结合在同一受体部位，因此标记的人绒毛膜促性腺激素被用来评估促黄体生成素结合部位。每组幼鼠30~60只，取6只大鼠睾丸匀浆，制备27000×g睾丸间质细胞匀浆。从总共35只成年大鼠中，每27000×g制剂汇集一只大鼠的睾丸。测定了每个匀浆池的hCG和hPRL的结合特性。每个治疗组5~10个未成熟池和5~8个成人池。在未成熟大鼠，每天1、2.5、5、10或2 0μg oPRL sc连续2天，hCG结合位点分别增加到2 91、318、876、6 42和591fmolhCG/mg蛋白，而生理盐水对照组为2 12 fmolhCG/mg蛋白。受体亲和力仍然很强。
Various doses of ovine PRL (oPRL; NIH-P-S12) were injected into immature (22 days old) and adult (82 days old) Sprague-Dawley rats at 1400 h for 2 days to determine the effects of PRL on hCG- and hPRL-binding sites in Leydig cell homogenates. Since hCG and LH bind to the same receptor site, labeled hCG was used for the assessment of LH-binding sites. From a total of 30–60 immature rats for each treatment group, testes from 6 rats were pooled for homogenization and preparation of 27,000 × g Leydig cell homogenates. From a total of 35 adult rats, testes from single rats were pooled for each 27,000 × g preparation. Binding characteristics for hCG and hPRL were determined for each homogenate pool, viz. 5–10 immature and 5–8 adult pools per treatment group. In immature rats, 1, 2.5, 5, 10, or 20 μg oPRL sc/day for 2 days resulted in an increase in hCG-binding sites to 291, 318, 876, 642, and 591 fmol hCG/mg protein compared to 212 fmol hCG/mg protein in saline injected controls. Receptor affinity remained unch...