Keratorefractive effect of microwave keratoplasty on human corneas
Keratorefractive effect of microwave keratoplasty on human corneas
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DOI：
10.1016/j.jcrs.2009.10.032
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发表时间：
2010-03-01
影响因子：
2.8
通讯作者：
Marshall, John
中科院分区：
文献类型：
--
作者：
Barsam, Allon;Patmore, Anne;Marshall, John
目得：为了确定微波角膜移植术应用后切除的人角巩膜纽扣上的屈光度变化，并确定使用组织学和扫描电子显微镜对角膜的定性影响，设置：雷恩研究所，圣托马斯医院，伦敦，英国学术眼科部。使用6.0 mm直径的内导体和8.4 mm直径的外导体在角膜上进行28.12 W、1秒的施加。采用TMS-1型角膜地形图仪对微波角膜移植术前后的角膜进行摄像。使用距离角膜几何顶点2.0 - 6.0 mm的环的平均屈光度计算角膜曲率的诱导变化。扫描电子显微镜和甲苯胺蓝光显微镜进行，以确定对角膜stroma.RESULTS的效果：6个切角巩膜纽扣从6个供体。应用微波角膜移植术后的平均曲率降低为3.07屈光度+/- 2.62（SD）。扫描和光学显微镜显示微波诱导的角膜基质胶原蛋白的收缩与小干扰上覆epitheli.CONCLUSION：微波角膜移植术降低角膜曲率和治疗潜力作为一种非侵入性的替代准分子激光手术矫正近视和作为一种治疗角膜扩张。
PURPOSE: To determine the change in dioptric power on excised human corneoscleral buttons after microwave keratoplasty application and to determine the qualitative effect on the cornea using histology and scanning electron microscopy.SETTING: Department of Academic Ophthalmology, Rayne Institute, St. Thomas' Hospital, London, United Kingdom.METHODS: Excised human corneoscleral buttons were treated with a prototype microwave keratoplasty ring applicator. A 28.12 W, 1-second application was performed on the corneas using a 6.0 mm diameter inner conductor and an 8.4 mm diameter outer conductor. Videokeratography was performed with a topographic modeling system (TMS-1) before and after microwave keratoplasty. The induced change in corneal curvature was calculated using the mean dioptric power of rings situated 2.0 to 6.0 mm from the geometrical apex of the corneas. Scanning electron microscopy and toluidine-blue light microscopy were performed to determine the effect on the corneal stroma.RESULTS: Six excised corneoscleral buttons from 6 donors were used. The mean reduction in curvature after microwave keratoplasty application was 3.07 diopters +/- 2.62 (SD). Scanning and light microscopy showed microwave-induced shrinkage of corneal stromal collagen with little disturbance to the overlying epithelium.CONCLUSION: Microwave keratoplasty reduced corneal curvature and has therapeutic potential as a noninvasive alternative to excimer laser surgical correction of myopia and as a treatment for corneal ectasia.