Role of colony‐stimulating factors in osteoclast development
Role of colony‐stimulating factors in osteoclast development
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集落刺激因子在破骨细胞发育中的作用
DOI：
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发表时间：
1991
影响因子：
6.2
通讯作者：
T. Suda
中科院分区：
文献类型：
--
作者：
N. Takahashi;N. Udagawa;T. Akatsu;Hirofumi Tanaka;M. Shionome;T. Suda
在两种不同的培养体系中：一步法小鼠骨髓培养体系和两步法小鼠原代成骨细胞共培养体系中，观察了不同的集落刺激因子[白介素3(IL-3)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)、巨噬细胞集落刺激因子(M-CSF)和粒细胞集落刺激因子(G-CSF)]对破骨细胞样细胞形成的影响。在一步法小鼠骨髓培养中，CSF均不能刺激破骨细胞的标志酶--抗酒石酸酸性磷酸酶(TRAP)阳性的多核细胞(MNC)的形成。此外，CSF对1α-25-二羟基维生素D3[1α，25-(OH)2D3]诱导的TRAP阳性单核细胞的形成具有剂量依赖性抑制作用。相反，当骨髓细胞首先在有脑脊液的半固体甲基纤维素中培养，然后从半固体培养中回收的骨髓细胞与原代成骨细胞在1α，25-(OH)2D3的存在下共培养时，形成了大量TRAP阳性的单核细胞。[125I]鲑鱼降钙素与在该两步培养系统中形成的TRAP阳性细胞特异性结合。90%以上的TRAP阳性的单核细胞和MNC积聚了[125I]降钙素。M-CSF诱导TRAP阳性的MNC的能力最强，其次是GM-CSF、IL-3和G-CSF。在没有成骨细胞和1-α，25-(OH)2D3的情况下，没有形成TRAP阳性细胞。这些结果清楚地表明，CSF，尤其是M-CSF能够刺激破骨细胞前体细胞的生长，在成骨细胞存在的情况下，1-α，25-(OH)2D3能刺激破骨细胞前体细胞分化为破骨细胞样细胞。
Effects of various colony‐stimulating factors (CSFs) [interleukin‐3 (IL‐3), granulocyte‐macrophage CSF (GM‐CSF), macrophage CSF (M‐CSF), and granulocyte CSF (G‐CSF)] on osteoclast‐like cell formation were examined in two different culture systems: the one‐step mouse marrow culture system and the two‐step coculture system of mouse primary osteoblastic cells with the bone marrow cells collected from the colonies that formed in the methylcellulose in the presence of the CSFs. In the one‐step mouse marrow cultures, none of the CSFs stimulated the formation of tartrate‐resistant acid phosphatase (TRAP, a marker enzyme of osteoclasts)‐positive multinucleated cells (MNCs). Furthermore, the CSFs other than G‐CSF inhibited in a dose‐dependent manner the TRAP‐positive MNC formation induced by 1α‐25‐dihydroxyvitamin D3 [1α,25‐(OH)2D3]. In contrast, when marrow cells were first cultured in semisolid methylcellulose in the presence of a CSF and the recovered marrow cells from the semisolid cultures were subsequently cocultured with primary osteoblastic cells in the presence of 1α,25‐(OH)2D3, numerous TRAP‐positive MNCs were formed. [125I]salmon calcitonin specifically bound to TRAP‐positive cells formed in this two‐step culture system. Over 90% of the TRAP‐positive mononuclear cells and MNCs accumulated [125I]calcitonin. M‐CSF was the most potent in inducing TRAP‐positive MNCs, followed by GM‐CSF, IL‐3, and G‐CSF in that order. No TRAP‐positive cells were formed in the absence of either osteoblastic cells or 1α,25‐(OH)2D3. These results clearly indicate that the CSFs, especially M‐CSF, stimulate the growth of osteoclast progenitors, which differentiate into osteoclast‐like cells in response to 1α,25‐(OH)2D3 in the presence of osteoblastic cells.
集落刺激因子调节来自体外最近复制的细胞的多核破骨细胞的发育。
DOI：
10.1172/jci113042
发表时间：
1987
期刊：
The Journal of clinical investigation
影响因子：
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作者：
Lorenzo,JA;Sousa,SL;Fonseca,JM;Hock,JM;Medlock,ES
通讯作者：
Medlock,ES
DOI：
10.1073/pnas.87.12.4828
发表时间：
1990-06-01
影响因子：
11.1
作者：
WIKTORJEDRZEJCZAK, W;BARTOCCI, A;STANLEY, ER
通讯作者：
STANLEY, ER