Studies on the dynamics of experimental transmission of West Nile virus by Culex molestus.
Studies on the dynamics of experimental transmission of West Nile virus by Culex molestus.
复制标题
坏库蚊实验性传播西尼罗河病毒动态的研究。
DOI：
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发表时间：
1955
影响因子：
3.3
通讯作者：
N. Goldblum
中科院分区：
文献类型：
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作者：
A. S. Tahori;V. Sterk;N. Goldblum
总结
骚扰库蚊通过取食西尼罗病毒悬液感染，并将病毒传播给易感动物。使用了最近从以色列西尼罗河热患者血液中分离出的病毒株。当悬浮液的滴度为10-4.0或更高时，在7-28天的外源性孵育期后，病毒通过蚊子传播给幼鼠。当滴度为10-2.0或10-2.8时，即使蚊子中存在病毒，也不会发生传播。被叮咬感染的小鼠百分比似乎是外源性潜伏期长度的函数。
在不同的实验中，感染蚊子体内的病毒滴度变化很大，在潜伏期的第9天和第28天之间在10-2.2和10-5.7之间波动。发现受感染的蚊子池可由病毒滴度在小于10-1.0至10-3.5范围内的个体蚊子组成。虽然每只蚊子在10-5.0或更高滴度的悬浮液中进食都会被感染，但只有一半的感染者能够在适当的潜伏期后通过叮咬传播感染。发现通过感染蚊子的叮咬注射到幼小鼠中的病毒量对应于约1000 ID 50。
Summary
Culex molestus was infected by feeding on suspensions of West Nile virus and the virus transmitted to susceptible animals. Strains of virus recently isolated from the blood of West Nile fever patients in Israel were used. When the titer of the suspension fed was 10-4.0 or more, virus was transmitted by mosquitoes to infant mice after an extrinsic incubation period of 7–28 days. When the titer was 10-2.0 or 10-2.8, transmission did not occur, even though virus was present in the mosquitoes. The percentage of mice infected by bite seemed to be a function of the length of the extrinsic incubation period.
The titer of virus in infected mosquitoes varied considerably in different experiments and fluctuated between 10-2.2 and 10-5.7 between the 9th and 28th day of the incubation period. It was found that an infected mosquito pool may be composed of individual mosquitoes having titers of virus ranging from less than 10-1.0 to 10-3.5. Though each mosquito feeding on a suspension having a titer of 10-5.0 or more became infected, only half of those infected were able to transmit the infection by bite after a suitable incubation period. The amount of virus injected into infant mice by the bite of infected mosquitoes was found to correspond to about 1000 ID50.