Evaluation of an IgG enzyme-linked immunosorbent assay for dengue diagnosis
Evaluation of an IgG enzyme-linked immunosorbent assay for dengue diagnosis
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DOI：
10.1016/s1386-6532(99)00059-1
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发表时间：
1999-12-01
影响因子：
8.8
通讯作者：
Vorndam, V
中科院分区：
文献类型：
--
作者：
Miagostovich, MP;Nogueira, RMR;Vorndam, V
背景：血凝抑制 (HI) 测试与 IgM 抗体捕获 ELISA (MAC-ELISA) 一起已成为诊断登革热病毒感染的标准之一。登革热在世界范围内的传播以及待检测病例数量的增加使得 IgG 抗体的 ELISA 形式检测取代 HI 检测变得非常必要。目标：评估 IgG-ELISA 作为 HI 测试替代品在登革热诊断中的使用情况。研究设计：通过 ELISA 检测 IgG 抗体，对定义为来自 HI 的原发性或继发性登革热病毒感染的配对血清样本进行测试。检查了这两项测试之间的滴度和血清学解释的相关性。结果：在原发感染中，IgG-ELISA与HI的相关性较低，而在继发感染中，由于HI检测中IgM抗体的影响，IgG-ELISA与HI的相关性较高。尽管如此，在症状出现后几天进行分析时，IgG ELISA 滴度可以可靠地与原发或继发感染相关，并且可用于表征黄病毒感染后的免疫反应。结论：IgM 和 IgG ELISA 的组合可用于血清学诊断登革热病毒感染，因为 IgG ELISA 可以替代 HI 测试来表征对登革热病毒感染的免疫反应。 (C) 1999 Elsevier Science B.V. 保留所有权利。
Background: The hemagglutination inhibition (HI) test has been one of the standards, with the IgM antibody capture ELISA (MAC-ELISA), for the diagnosis of dengue virus infections. The spread of dengue throughout the world and the increasing number of cases to be tested makes an ELISA-format test for IgG antibodies to replace the HI test highly desirable. Objectives: Evaluate the use of the IgG-ELISA as a substitute for the HI test in dengue diagnosis. Study design: Paired serum samples defined as being from primary or secondary dengue virus infections by HI, were tested by an ELISA that detects IgG antibodies. The correlations of titers and serologic interpretations between these two tests were examined. Results: The IgG-ELISA showed a low correlation with the HI in primary infections, and a higher correlation in secondary infections because of the influence of IgM antibodies in the HI test. Nevertheless, IgG ELISA titers could be reliably associated with primary or secondary infections when analyzed by days after onset of symptoms, and can be used to characterize the immune response after flavivirus infections. Conclusion: The combination of the IgM and IgG ELISAs may be used to serologically diagnose dengue virus infections, since the IgG ELISA can substitute for the HI test in characterizing the immune response to dengue virus infections. (C) 1999 Elsevier Science B.V. All rights reserved.