Proteomic identification and expression of oral apparatus constituents in cell regeneration of giant ciliate Stentor coeruleus (strain WHEL)
Proteomic identification and expression of oral apparatus constituents in cell regeneration of giant ciliate Stentor coeruleus (strain WHEL)
复制标题
巨型纤毛虫Stentor coeruleus（WHEL品系）细胞再生中口腔器成分的蛋白质组学鉴定和表达
DOI：
10.1016/j.gene.2020.144624
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发表时间：
2020-06-15
期刊：
影响因子：
3.5
通讯作者：
Xiong, Jie
中科院分区：
文献类型：
--
作者：
Wei, Wei;Jiang, Chuanqi;Xiong, Jie
蓝斑大喇叭虫Stentorcoeruleus（S.蓝斑）是在单细胞水平上研究形态发生和再生的合适模式生物。它在细胞的前端包含一个突出的结构，称为口腔器（OA）。尿素处理可诱导OA脱落，然后通过一系列确定的形态学事件再生新的OA，细胞恢复正常的摄食活动。我们在S.蓝斑核共鉴定出882种OA相关蛋白，其中181种的同源物是其他生物体中已知的OA成分。使用单细胞转录组测序研究再生过程中OA相关基因的表达模式。大多数OA相关基因的表达在再生过程中是高的，表明它们在OA脱落后稳定表达。我们还确定了OA相关的差异表达基因，可能参与调节OA重建。综上所述，本研究对S.蓝核
The giant ciliate Stentor coeruleus (S. coeruleus) is a suitable model organism for studying morphogenesis and regeneration at the single-cell level. It contains a prominent structure on the anterior end of the cell, known as the oral apparatus (OA). OA can be induced to shed by urea treatment and then new OA regenerates via a series of defined morphological events and the cell resumes normal feeding activity. We identified OA constituents in S. coeruleus by mass spectrometry. A total of 882 OA-associated proteins were identified; the homologs of 181 of these are known OA constituents in other organisms. The expression pattern of OA-associated genes during regeneration was investigated using single-cell transcriptome sequencing. The expression of most OA-associated genes was high during regeneration, indicating their stable expression after OA shedding. We also identified OA-associated differentially expressed genes that may be involved in regulating OA reconstruction. In summary, this study gives preliminary insight into the molecular basis of OA in S. coeruleus.