Sediment pH, not the bacterial diversity, determines Escherichia coli O157:H7 survival in estuarine sediments
Sediment pH, not the bacterial diversity, determines Escherichia coli O157:H7 survival in estuarine sediments
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沉积物 pH 值（而非细菌多样性）决定了大肠杆菌 O157:H7 在河口沉积物中的存活
DOI：
10.1016/j.envpol.2019.06.019
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发表时间：
2019-09-01
影响因子：
8.9
通讯作者：
Zhang, Demin
中科院分区：
文献类型：
--
作者：
Liang, Chunling;Yao, Zhiyuan;Zhang, Demin
志贺毒素产生菌O157：H7是公认的环境中的一种有害微生物。它的存活时间更长，可能会导致更高的污染风险。本研究调查了杭州湾南部河口沉积物中O157：H7大肠杆菌的存活情况。大肠杆菌O157：H7在河口沉积物中的存活时间随距水陆交界处距离的增加而延长。沉积物pH是影响O157：H7大肠杆菌在河口沉积物中存活的最重要因素。此外，底泥营养物质和质地对O157：H7大肠杆菌在底泥中的存活也起着重要作用。另一方面，由α多样性指数确定的细菌多样性对大肠杆菌O157：H7的存活率没有显著影响。然而，特定的细菌家族与大肠杆菌O157：H7在沉积物中的存活密切相关。值得注意的是，一些主要参与硫循环的潜在细菌群，如脱硫杆菌科、脱硫球菌科和脱硫菌科，与沉积物中E.ColiO157：H7的存活率呈显著负相关。总体而言，非生物因素对大肠杆菌O157：H7在试验底泥中存活的影响大于细菌群落。我们的发现为全面了解O157：H7在河口沉积物中的存活和调控因素提供了依据，为预防病原体污染奠定了基础。(C)2019爱思唯尔有限公司。保留所有权利。
Shiga toxin-producing Escherichia coli (E. coli) O157:H7 is recognized as a hazardous microorganism in the environment. Its longer survival might contribute to higher contamination risk. In this study, E. coli O157:H7 survival in estuarine sediments collected from south Hangzhou Bay was investigated. The survival time of E. coli O157:H7 in estuarine sediments increased with the distance to the water-land junction. Sediment pH was the most important factor in regulating E. coli O157:H7 survival in estuarine sediments. In addition, sediment nutrients and texture also played significant roles in the survival of E. coli O157:H7 in the sediments. On the other hand, bacterial diversity as determined by the alphadiversity index had no significant effect on E. coli O157:H7 survival. However, specific families of bacteria were closely associated with E. coli O157:H7 survival in the sediments. Remarkably, some potential bacterial groups, e.g., the Desulfobacteraceae, Desulfobulbaceae and Desulfarculaceae families, which are mainly involved in the sulfur cycle, showed significant negative correlation with the E. coli O157:H7 survival in the sediments. On the whole, abiotic factors showed greater effects on E. coli O157:H7 survival in the test sediments than the bacterial community. Our findings provide a comprehensive understanding of E. coli O157:H7 survival and regulatory factors in estuarine sediments, establishing foundation for the prevention of pathogen contamination. (C) 2019 Elsevier Ltd. All rights reserved.