Immunocytochemical localization of androgen receptors in human skin using monoclonal antibodies against the androgen receptor.
Immunocytochemical localization of androgen receptors in human skin using monoclonal antibodies against the androgen receptor.
复制标题
DOI：
10.1111/1523-1747.ep12472330
复制
发表时间：
1993-05
期刊：
影响因子：
--
通讯作者：
Tehming Liang;Sheryl Hoyer;Riley Yu;Keyoumars Soltani;Allan L. Lorincz;R. Hiipakka;S. Liao
中科院分区：
文献类型：
--
作者：
Tehming Liang;Sheryl Hoyer;Riley Yu;Keyoumars Soltani;Allan L. Lorincz;R. Hiipakka;S. Liao
使用抗人雄激素受体的单抗，在人体皮肤的冰冻切片中定位雄激素受体。以生物素标记的兔抗鼠免疫球蛋白、过氧化物酶标记的链霉亲和素和二氨基联苯胺为显色原检测结合抗体。在新生儿包皮中，雄激素受体抗体与表皮中的角质形成细胞以及真皮中的成纤维细胞和血管内皮细胞结合。免疫组织化学染色胞核强于胞浆。这种染色是特异的，因为当雄激素受体抗体被来自未免疫的大鼠或用缓冲液替代时，或者当雄激素受体抗体在免疫染色前与Trp E-人雄激素-受体融合蛋白作为免疫原孵育时，没有明显的染色。雄激素受体抗体单独与Trp E孵育不影响染色。雄激素受体抗体也与成年男性和女性皮肤中的角质形成细胞、成纤维细胞和内皮细胞结合。头皮、鼻子、嘴唇、背部和胸部的皮肤雄激素受体染色呈阳性。雄激素受体抗体还可结合到内分泌腺的卷曲和导管细胞、毛囊外根鞘、毛球上皮、毛乳头细胞和皮脂细胞。与脂肪细胞、胶原蛋白或角质层无明显结合。这些结果表明，雄激素受体存在于已知为雄激素靶标的细胞中，也存在于雄激素的生物效应尚不清楚的细胞中。
Androgen receptors were localized in cryostat sections of human skin using monoclonal antibodies to the human androgen receptor. Bound antibodies were detected using biotinylated rabbit anti-rat IgG, peroxidase-conjugated streptavidin, and diaminobenzidine as chromogen. In the neonatal foreskin, antibody to androgen receptor bound to keratinocytes in the epidermis and to fibroblasts and vascular endothelial cells in the dermis. Immunohistochemical staining was stronger in nuclei than in cytoplasm. This staining was specific, because there was no significant staining when antibody to the androgen receptor was replaced with IgG from nonimmunized rats or with buffer, or when antibody to androgen receptor was incubated, prior to immunostaining, with a trp E-human androgen-receptor fusion protein used as immunogen. Incubation of androgen receptor antibody with trp E alone did not affect staining. Androgen-receptor antibody also bound to keratinocytes, fibroblasts, and endothelial cells in skin from adult men and women. Skin from the scalp, nose, lip, back, and chest gave positive staining for androgen receptor. Antibody to androgen receptor also bound to the coil and ductal cells of eccrine glands, external root sheath of hair follicles, epithelium in the hair bulb, dermal papilla cells, and sebocytes. There was no significant binding to adipocytes, collagen, or stratum corneum. These results show that androgen receptor is present in cells that are known to be targets for androgens and also in cells in which the biologic effects of androgens are yet to be characterized.