Dosage and kinetics of MMC release of a collagen implant used as a delivery device in glaucoma surgery in the rabbit eye
Dosage and kinetics of MMC release of a collagen implant used as a delivery device in glaucoma surgery in the rabbit eye
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DOI：
10.1016/s0181-5512(06)73893-1
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发表时间：
2006-11-01
影响因子：
1.2
通讯作者：
Drewe, J.
中科院分区：
文献类型：
--
作者：
Adjadj, E.;Roy, S.;Drewe, J.
目的：为了确定的吸收和释放丝裂霉素-C从胶原蛋白植入物和组织浸渍在前面的结构中的兔eye.Methods：确定丝裂霉素-C的量，胶原蛋白植入物可以吸收与干燥和浸泡重量之间的差异。使用分光光度法在体外测量丝裂霉素-C释放。使用生物测定重复测量。对6只家兔的12只眼进行了眼组织浸润检查。巩膜，植入物，水，睫状体标本收集的浓度测量使用HPLC从1至6小时后surgical.Results：平均丝裂霉素-C的量吸收在植入物为3.22 +/- 0.2 μ g。体外释放10 min后为0.13 mg/ml，6 h后为0.05 μ g/ml。生物活性测定结果显示，巩膜中几乎没有抗纤维化活性。结论：滤过性手术后，胶原植入物中丝裂霉素C释放明显，眼组织得到有效浸润。
Purpose: To determine the absorption and the release of mitomycin-C from a collagen implant and tissue impregnation in the anterior structures of the rabbit eye.Methods: Determining the quantity of mitomycin-C that a collagen implant can absorb with the difference between dry and soaked weight. Mitomycin-C release was measured in vitro using spectrophotometry. The measurement was repeated using a bioassay. Ocular tissue impregnation was determined in 12 eyes of six rabbits. Sclera, implant, aqueous, and ciliary body specimens were collected for concentration measurement using HPLC from 1 to 6 h after surgery.Results: The mean mitomycin-C quantity absorbed in the implant was 3.22 +/- 0.2 mu g. In vitro release was 0.13 mg/ml after 10 min and 0.05 mu g/ml at 6 h. The bioassay showed almost no antifibrotic activity in sclera. In vivo release of mitomycin-C was high from the first to the sixth hour.Conclusion: After filtering surgery, mitomycin-C in the collagen implant is clearly released and ocular tissues are effectively impregnated.