Identification of a novel hydrogen producing bacteria from sugarcane bagasse waste
Identification of a novel hydrogen producing bacteria from sugarcane bagasse waste
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DOI：
10.1016/j.bcab.2018.07.003
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发表时间：
2018-07-01
影响因子：
4
通讯作者：
Jonesh, S.
中科院分区：
文献类型：
--
作者：
Dhanasekar, R.;Jonesh, S.
从甘蔗渣堆放场土壤中筛选到一株纤维素降解能力较强的产氢菌。在筛选的菌落中，新鉴定的菌株枯草芽孢杆菌AuChE 413在产氢率和纤维素降解方面表现出更好的性能。对分离菌株进行了形态学、生理生化特性的研究。并通过16 S rRNA基因序列分析，研究了该菌的分子特征。将枯草芽孢杆菌AuChE 413的基因序列与GenBank中的其他菌株的基因序列进行BLASTn比对，分析其与其他物种的同源性。采用邻接法构建系统发育树。以预处理后的SCB和甜高粱秸秆生物质（SSB）为底物，测定了枯草芽孢杆菌AUChE 413的产氢能力。相比之下，底物高粱秸秆在pH 7和37 ℃下产生了最高的氢气产量，为55.21 H-2/kg SSB。
A new hydrogen producing strain with potent cellulose degrading ability was isolated from soil sample collected from sugarcane bagasse (SCB) storage yard. Among the colonies screened, the newly identified strain, Bacillus subtilis AuChE413 has shown better performance in terms of hydrogen yield and cellulose degradation. Morphological, physiological, and biochemical traits of the isolated strain were studied. And its molecular characterization was studied by analyzing the sequence of 16S rRNA gene. The sequence of Bacillus subtilis AuChE413 was compared with other GenBank sequences using BLASTn to study its homology with other species. Phylogenetic tree was constructed using the neighbor joining method. The hydrogen production capacity of Bacillus subtilis AUChE413 was tested by employing the pretreated SCB and sweet sorghum stalk biomass (SSB) as substrates. In comparison, the substrate sorghum stalk has produced the highest hydrogen yield of 55.21 H-2/kg SSB at pH 7 and 37 degrees C.