Mesenchymal stem cells from the outer ear: a novel adult stem cell model system for the study of adipogenesis
Mesenchymal stem cells from the outer ear: a novel adult stem cell model system for the study of adipogenesis
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DOI：
10.1096/fj.04-3204fje
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发表时间：
2005-04-01
期刊：
影响因子：
4.8
通讯作者：
Gawronska-Kozak, B
中科院分区：
文献类型：
--
作者：
Rim, JS;Mynatt, RL;Gawronska-Kozak, B
脂肪细胞起源于中胚层起源的多能干细胞，它也产生肌肉、骨骼和软骨谱系。然而，由于缺乏适当的模型系统，使多能干细胞进入脂肪细胞谱系的信号和早期分子事件尚未得到很好的确定。我们已经确定了一种来自小鼠外耳的成体干细胞的新来源，这里称为耳间充质干细胞（EMSC）。EMSC已从几种标准和突变小鼠株中分离出来。它们具有自我更新、克隆性和多能性，因为它们产生骨细胞、软骨细胞和脂肪细胞。体外表征表明，EMSC非常容易分化成脂肪。诱导分化后的形态学、组织化学和分子分析表明，EMSC维持成脂潜能至第5代。在相同的培养条件下（异丁基甲基黄嘌呤、地塞米松和胰岛素），EMSC与脂肪库的基质-血管（S-V）部分的比较显示，EMSC中的脂肪生成比S-V部分更加强劲和一致。总之，我们表明EMSC可以为脂肪形成的研究提供一种新的、容易获得的原代培养模型。
Adipocytes arise from multipotent stem cells of mesodermal origin, which also give rise to the muscle, bone, and cartilage lineages. However, signals and early molecular events that commit multipotent stem cells into the adipocyte lineage are not well established mainly due to lack of an adequate model system. We have identified a novel source of adult stem cells from the external murine ears referred to here as an ear mesenchymal stem cells (EMSC). EMSC have been isolated from several standard and mutant strains of mice. They are self-renewing, clonogenic, and multipotent, since they give rise to osteocytes, chondrocytes, and adipocytes. The in vitro characterization of EMSC indicates very facile adipogenic differentiation. Morphological, histochemical, and molecular analysis after the induction of differentiation showed that EMSC maintain adipogenic potentials up to fifth passage. A comparison of EMSC to the stromal-vascular (S-V) fraction of fat depots, under identical culture conditions (isobutyl-methylxanthine, dexamethasone, and insulin), revealed much more robust and consistent adipogenesis in EMSC than in the S-V fraction. In summary, we show that EMSC can provide a novel, easily obtainable, primary culture model for the study of adipogenesis.