DEVELOPMENTAL DISTURBANCE OF RAT CEREBRAL-CORTEX FOLLOWING PRENATAL LOW-DOSE GAMMA-IRRADIATION - A QUANTITATIVE STUDY
DEVELOPMENTAL DISTURBANCE OF RAT CEREBRAL-CORTEX FOLLOWING PRENATAL LOW-DOSE GAMMA-IRRADIATION - A QUANTITATIVE STUDY
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DOI：
10.1016/0014-4886(91)90129-z
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发表时间：
1991-06-01
影响因子：
5.3
通讯作者：
KAMEYAMA, Y
中科院分区：
文献类型：
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作者：
FUKUI, Y;HOSHINO, K;KAMEYAMA, Y
妊娠大鼠在妊娠第15天接受单次全身γ射线照射，剂量为0.27、0.48、1.00或1.46戈伊。让它们分娩，并在6或12周龄时处死后代，对大脑进行显微镜和电子显微镜检查。测量大鼠体重、脑重、皮层厚度、体感皮层全细胞数密度和突触数密度。还用Golgi-Cox标本定量检查了第V层锥体细胞的树突状分支的生长。在所有照射组中发现脑重量的显著剂量相关减少。在0.27戈伊照射组中未观察到大体畸形或皮质结构异常。在0.48戈伊及以上剂量组，皮质厚度有明显变化。戈伊照射组细胞堆积密度明显增加。在暴露于0.27戈伊或更高剂量的组中，发现树突与带状边界的交叉点数量显著减少。对照组和照射组间I层突触数密度无差异。这些结果表明，低至0.27戈伊的剂量可引起哺乳动物大脑形态学上可辨别的变化。
Pregnant rats were exposed to a single whole-body γ-irradiation on Day 15 of gestation at a dose of 0.27, 0.48, 1.00, or 1.46 Gy. They were allowed to give birth and the offspring were killed at 6 or 12 weeks of age for microscopic and electron microscopic examinations of the cerebrum. Their body weight, brain weight, cortical thickness, and numerical densities of whole cells and synapses in somatosensory cortex were examined. Growth of the dendritic arborization of layer V pyramidal cells was also examined quantitatively with Golgi-Cox specimens. A significant dose-related reduction in brain weight was found in all irradiated groups. Neither gross malformation nor abnormality of cortical architecture was observed in the groups exposed to 0.27 Gy. A significant change was found in thickness of cortex in the groups exposed to 0.48 Gy or more. Cell packing density increased significantly in the group exposed to 1.00 Gy. Significant reduction in the number of intersections of dendrites with the zonal boundaries were found in the groups exposed to 0.27 Gy or more. There was no difference in the numerical density of synapses in layer I between the control and irradiated groups. These results suggested that doses as low as 0.27 Gy could cause a morphologically discernible change in the mammalian cerebrum.