Nanofibers of Silk Fibroin Controlled by the Crystallization of Polyethylene Glycol inFrozen Solution
Nanofibers of Silk Fibroin Controlled by the Crystallization of Polyethylene Glycol inFrozen Solution
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冷冻溶液中聚乙二醇结晶控制丝素纳米纤维
DOI：
10.1007/s10118-017-1974-x
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发表时间：
2017
影响因子：
4.3
通讯作者：
Ping Zhou
中科院分区：
文献类型：
--
作者：
Congheng Chen;Juan Zhao;Zhou Yang;Qing Sun;Ping Zhou
用光谱和显微镜方法研究了分子量为2 ~ 20 kDa的聚乙二醇（PEG）与丝素蛋白（SF）质量比为1：5 ~ 10：1的丝素蛋白（SF）的冷冻构象。结果表明，在-20 °C时，PEG结晶产生的应力可以诱导SF从无规卷曲向β-折叠的构象转变，并与降温速率、PEG：SF质量比和PEG分子量密切相关。这些发现为制备所需的SF纳米纤维提供了一种新的方法。
The conformation of silk fibroin (SF) frozen with polyethylene glycol (PEG) at a molecular weight from 2 kDa to 20 kDa and a mass ratio of PEG:SF from 1:5 to 10:1 was studied by spectral and microscopic methods. It is found that the conformation transition of SF from random coil toβ-sheet could be induced by the stress resulting from PEG crystallization at −20 °C, and greatly depended on the cooling rate, PEG:SF mass ratio and PEG molecular weight. These findings provide a new method for the preparation of desired SF nanofibers.