Analysis of specific IgE and IgG subclass antibodies for diagnosis of Echinococcus granulosus
Analysis of specific IgE and IgG subclass antibodies for diagnosis of Echinococcus granulosus
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DOI：
10.1111/j.1365-3024.2006.00837.x
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发表时间：
2006-08-01
影响因子：
2.2
通讯作者：
Siavashi, M. R.
中科院分区：
文献类型：
--
作者：
Khabiri, A. R.;Bagheri, F.;Siavashi, M. R.
应用以抗原5(AG5)为基础的酶联免疫吸附试验(EL ISA)检测不同免疫球蛋白G亚类抗体和IgE在囊型包虫病血清学诊断中的作用。58例CE患者血清中均检出IgG1。在这项研究中发现的最具区别性和特异性的抗体属于IgG4和IgE。40份健康对照血清中仅有1份IgG4假阳性反应，未发生IgE交叉反应。在36份非CE寄生虫感染者血清中，发现两份IgG4假阳性反应，而IgE均未见阳性反应。在免疫印迹中，免疫印迹显示IgG1亚类负责与AG5的38 kDa亚基反应的人抗体的交叉反应。IgG4和IgE抗体不识别38 kDa亚基，在非还原条件下与57 kDa亚基发生反应，与其他寄生虫无交叉反应。结果表明，在以AG5为基础的免疫检测系统中，IgG4和IgE是诊断包虫病最重要的抗体。
The potential roles of specific antibodies of different immunoglobulin G (IgG) subclasses and IgE in serological diagnosis of cystic echinococcosis (CE) were investigated by an enzyme linked immunosorbent assay (ELISA) based on Antigen 5 (Ag5). Presence of IgG1 was demonstrated in all sera from 58 patients with CE. The most discriminatory and specific antibodies found in this study belonged to IgG4 and IgE. Only one false-positive reaction was observed with IgG4 and no IgE cross-reactivity occurred with 40 sera from healthy controls. In 36 sera from patients infected with parasites other than CE two false-positive reactions with IgG4 were observed but none occurred with IgE. In immunoblotting, it was shown that IgG1 subclass was responsible for cross-reactivity of human antibodies that reacted with a 38 kDa subunit of Ag5. IgG4 and IgE antibodies could not recognize the 38 kDa subunit and under non-reducing conditions reacted with the 57 kDa subunit without any cross-reactivity to other parasites. The results demonstrated that IgG4 and IgE are the most important antibodies for serological diagnosis of hydatid cyst in an Ag5 based immunoassay system.