New cellular models for tracking the odontoblast phenotype
New cellular models for tracking the odontoblast phenotype
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DOI：
10.1016/j.archoralbio.2004.10.007
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发表时间：
2005-02-01
影响因子：
3
通讯作者：
Poliard, A
中科院分区：
文献类型：
--
作者：
Priam, F;Ronco, V;Poliard, A
牙科医生和骨科医生在功能和组织上是不同的。牙源性间充质细胞与骨源性间充质细胞关系密切，在体外很难区分。事实上，成骨细胞外基质的主要非胶原性成分，牙本质唾液蛋白(DSP)或牙本质基质蛋白1(DMP1)，也在成骨细胞中被检测到。发展成牙本质细胞细胞模型的必要性。分化和鉴定成牙本质细胞系的特异性标记，使我们从18天小鼠胚胎的牙胚中建立了克隆细胞系，并将其转化为腺病毒-SV40重组质粒。在本研究中，我们用RT-PCR和Western Not分析了三个独立克隆的表型。这些克隆合成了DSP、DMPI和其他典型的成牙本质细胞外基质蛋白，因此很可能来自牙髓。编码一组与颅面发育有关的同源框蛋白的转录本，如Pax9、Msx1、Cbfa1、D1x2和5也得到了表达，尽管表达水平不同。牙髓克隆的这些特征被能够沿成骨、成软骨或成脂谱系分化的C1中胚层细胞所共享。相比之下，两个LIM结构域同源框家族基因(Lhx6和Lhx7)的转录本仅在牙齿克隆中检测到。由于这些基因在发育中的牙齿间充质中优先表达，这表明我们的转基因衍生细胞系保留了成牙本质细胞的固有特性。它们可能有助于确定指定成牙本质细胞表型和成牙本质细胞分化的信号通路的基因。(C)2004爱思唯尔有限公司。保留所有权利。
Odontobtasts and osteobtasts differ functionally and histologically. Because of their close relationship, mesenchymal cells derived from teeth and bone are difficult to distinguish ex vivo. Indeed, the main non-collagenous components of the oclontoblastic extracellular matrix, dentin sialoprotein (DSP) or dentin matrix protein 1 (DMP1), have also been detected in osteobtasts. The need to develop cellular models of odontoblast. differentiation and to identify markers specific for the odontoblast lineage, has led us to establish clonal cell lines f rom tooth germs of day 18 mouse embryos transgenic for an adenovirus-SV40 recombinant plasmid. In this study, we analyzed the phenotypes of three independent clones by RT-PCR and Western Not. These clones synthesised DSP, DMPI and other extracellular matrix proteins typical of the odontoblast and are therefore likely to be derived from the pulp. Transcripts encoding a set of homeobox proteins involved in craniofacial development, such as Pax9, Msx1, Cbfa1, D1x2 and 5 were also expressed albeit at a different level.. These features of the pulpal clones are shared by the C1 mesodermal cells that are capable of differentiating along osteogenic, chondrogenic or adipogenic lineages In contrast, transcripts for two LIM-domain homeobox family genes (Lhx6 and Lhx7) were only detected in the dental clones. Since these genes are preferentially expressed in the mesenchyme of the developing tooth, this suggests that our transgenic-derived cell tines retain intrinsic properties of odontoblastic cells. They may help to characterise genes specifying the odontoblast phenotype and the signalling pathways underlying odontobtast differentiation. (c) 2004 Elsevier Ltd. All rights reserved.