Immunological properties of conjugates of ragweed pollen antigen E with methoxypolyethylene glycol or a copolymer of D-glutamic acid and D-lysine.
Immunological properties of conjugates of ragweed pollen antigen E with methoxypolyethylene glycol or a copolymer of D-glutamic acid and D-lysine.
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DOI：
10.1084/jem.149.2.424
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发表时间：
1979-02-01
期刊：
影响因子：
--
通讯作者：
Chiorazzi N
中科院分区：
文献类型：
--
作者：
King TP;Kochoumian L;Chiorazzi N
豚草花粉的主要过敏原抗原E通过将其氨基酸基团与甲醇、5，000道尔顿的甲氧基聚乙二醇（MPEG）或34，000道尔顿的D-谷氨酸和D-赖氨酸（DGL）的合成共聚物偶联而进行修饰，所有这些都适当活化。结合物进行了化学和免疫学表征。与天然抗原相比，甲氧基偶联物显示出变应原活性几乎没有降低，但其他两种偶联物显示出强烈的降低，如通过用鼠抗抗原E反应素血清致敏的大鼠中的异源被动皮肤过敏反应所测量的。MPEG缀合物在已知对天然抗原有高应答的小鼠中显然是非免疫原性的。MPEG和DGL缀合物保留了天然抗原的免疫抑制性质，因为用这两种缀合物皮下处理抗原E致敏小鼠导致其抗原E特异性IgE和IgG抗体水平的显著持久抑制。这些免疫学变化被认为是由于大分子与蛋白抗原偶联后抗原价态和特异性降低所致。
The major allergen of ragweed pollen, antigen E, was modified by coupling its amino acid groups with either methanol, methoxypolyethylene glycol (MPEG) of 5,000 daltons, or a synthetic copolymer of D-glutamic acid and D-lysine (DGL) of 34,000 daltons, all appropriately activated. The conjugates were characterized chemically and immunologically. Compared to the native antigen, the methoxy conjugate showed little reduction in allergenic activity, but the other two conjugates showed strong reductions, as measured by heterologous passive cutaneous anaphylaxis in rats sensitized with murine anti- antigen E reaginic sera. The MPEG conjugate was apparently nonimmunogenic in mice known to be high responders to the native antigen. MPEG and DGL conjugates retained the immunosuppressive property of the native antigen as subcutaneous treatment of antigen E sensitized mice with these two conjugates led to significant long- lasting depression of their antigen E-specific IgE and IgG antibody levels. These immunological changes are believed to result from reduction of antigenic valency and specificity upon coupling the bulky molecules to the protein antigens.