Image analyzing method to evaluate in situ bioluminescence from an obligate anaerobe cultivated under various dissolved oxygen concentrations
Image analyzing method to evaluate in situ bioluminescence from an obligate anaerobe cultivated under various dissolved oxygen concentrations
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DOI：
10.1016/j.jbiosc.2012.09.006
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发表时间：
2013-02-01
影响因子：
2.8
通讯作者：
Shimizu, Nobuaki
中科院分区：
文献类型：
--
作者：
Ninomiya, Kazuaki;Yamada, Ryuji;Shimizu, Nobuaki
发展了一种图像分析方法来评估原位生物发光表达，而不需要将培养样品暴露在环境氧气中。利用这种方法，我们研究了溶解氧浓度对表达细菌荧光素酶的专性厌氧长双歧杆菌生物发光的影响，该酶催化需要氧气的生物发光反应。(C)2012年，日本生物技术学会。版权所有。
An image analyzing method was developed to evaluate in situ bioluminescence expression, without exposing the culture sample to the ambient oxygen atmosphere. Using this method, we investigated the effect of dissolved oxygen concentration on bioluminescence from an obligate anaerobe Bifidobacterium longum expressing bacterial luciferase which catalyzes an oxygen-requiring bioluminescent reaction. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.