N‐acetyl aspartate estimation: a potential method for determining neuronal loss in the transmissible spongiform encephalopathies
N‐acetyl aspartate estimation: a potential method for determining neuronal loss in the transmissible spongiform encephalopathies
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N-乙酰天冬氨酸估计：确定传染性海绵状脑病神经元损失的潜在方法
DOI：
10.1046/j.1365-2990.2003.00438.x
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发表时间：
2003
影响因子：
5
通讯作者：
J. Fraser
中科院分区：
文献类型：
--
作者：
Y;J. Barr;K. Bhakoo;S. Williams;Jimmy D Bell;J. Fraser
神经退行性病理学是传染性海绵状脑病 (TSE) 的典型特征，被认为是临床疾病的基础。一些形态测量研究显示了早期局灶性神经元损失，但 TSE 引起的中枢神经系统神经元损失的全部程度尚不清楚，只能使用强化形态测量技术来准确估计。我们使用了小鼠痒病模型，通过高效液相色谱和高分辨率质子磁共振波谱法测定了从海马体连续采集的样本中 N-乙酰天冬氨酸 (NAA)（一种假定的神经元标记物）的水平。该痒病模型在海马中出现严重的神经元损失，并且 NAA 水平与我们之前对神经元数量的形态测量估计值呈显着正相关。因此，NAA 测量可能为 TSE 神经变性研究中强化形态测量技术提供一种实用的替代方法。
Neurodegenerative pathology is typical of the transmissible spongiform encephalopathies (TSEs), and is thought to underlie clinical disease. Some morphometric studies have shown early focal neurone loss, but the full extent of TSE induced neuronal loss in the central nervous system is not known, and can only be accurately estimated using intensive morphometric techniques. We have used a murine scrapie model in which we determined the levels of N‐acetyl aspartate (NAA), a putative neuronal marker, by both high‐performance liquid chromatography and high resolution, proton magnetic resonance spectroscopy in samples taken sequentially from the hippocampus. This scrapie model develops severe neuronal loss in the hippocampus, and the NAA levels showed a significant positive correlation with our previous morphometric estimates of neurone number. NAA measurement may therefore provide a practical alternative to intensive morphometric techniques in the investigation of neurodegeneration in the TSEs.