Enantioselective cytotoxicity of the insecticide bifenthrin on a human amnion epithelial (FL) cell line
Enantioselective cytotoxicity of the insecticide bifenthrin on a human amnion epithelial (FL) cell line
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DOI：
10.1016/j.tox.2008.08.015
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发表时间：
2008-11-20
期刊：
影响因子：
4.5
通讯作者：
Liu, Weiping
中科院分区：
文献类型：
--
作者：
Liu, Huigang;Zhao, Meirong;Liu, Weiping
合成拟除虫菊酯（SP）由于其高效、对哺乳动物的低毒性和容易的生物降解性而优先于有机氯和有机磷酸酯使用。以往的研究报道，在水生生物毒性中存在着对映选择性毒性。一些研究表明，SP可导致人或动物的氧化损伤，这与其毒性作用有关。关于手性SP的单个立体异构体诱导的慢性毒性效应的差异知之甚少。本研究通过对联苯菊酯（BF）对人羊膜上皮细胞（FL）的毒性、遗传毒性和杀虫活性的对映体选择性研究，结果表明，当BF浓度超过7.5mg L-1时，1 S-cis-BF的毒性作用明显大于1 R-cis-BF（p > 0.05）。FL细胞与1 S-cis-BF孵育后，细胞内活性氧（ROS）呈剂量依赖性积累。在彗星实验中，1 S-cis-BF组DNA损伤细胞数明显多于1 R-cis-BF组（p < 0.01）。对映体对菜青虫的LC 50值分别为：表明1 R-cis-BF比1 S-cis-BF活性高300倍。这些结果表明，BF在非靶生物和靶生物之间的对映体选择性毒性和活性是可逆的。因此，在评价手性中心农药的环境安全性和开发新农药时，应考虑对映选择性。皇冠版权所有（c）2008由爱思唯尔爱尔兰有限公司出版。保留所有权利。
Synthetic pyrethroids (SPs) are used in preference to organochlorines and organophosphates due to their high efficiency, low toxicity to mammals, and ready biodegradability. Previous studies reported that enantioselective toxicity of SPs occurs in aquatic toxicity. Several studies have indicated that SPs could lead to oxidative damage in humans or animals which was associated with their toxic effects. Little is known about the differences in the effects of chronic toxicity induced by individual stereoisomers of chiral SPs. The present study was therefore undertaken to evaluate the enantioselectivity in cytotoxicity, genotoxicity caused by bifenthrin (BF) on human amnion epithelial (FL) cell lines and pesticidal activity on target organism.The cell proliferation and cytoflow analysis indicated that 1S-cis-BF presented more toxic effects than 1R-cis-BF above the concentration of 7.5 mg L-1 (p > 0.05). FL cells incubated with 1S-cis-BF exhibited a dose-dependent accumulation of intracellular reactive oxygen species (ROS). In the comet assay, the number of cells with damaged DNA incubated with 1S-cis-BF was more than that with 1R-cis-BF (p < 0.01). While the LC50 values of enantiomer to the target pest on Pieris rapae L. show that 1R-cis-BF was 300 times more active than 1S-cis-BF. These results indicate that the enantioselective toxicity and activity of BF between non-target organism and target organism was reversal. These implications together suggest that assessment of the environmental safety and new pesticides development with chiral centers Should consider enantioselectivity. Crown Copyright (c) 2008 Published by Elsevier Ireland Ltd. All rights reserved.