Protective effect of compounds from the flowers of Citrus aurantium L. var. amara Engl against carbon tetrachloride-induced hepatocyte injury.
Protective effect of compounds from the flowers of Citrus aurantium L. var. amara Engl against carbon tetrachloride-induced hepatocyte injury.
复制标题
枳壳花化合物的保护作用
DOI：
10.1016/j.fct.2013.08.041
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Xi
中科院分区：
文献类型：
--
作者：
Q. Lu;Li Yang;Haifeng Zhao;Jian;Xi
5-羟基-6，7，3 ′，4 ′-四甲氧基黄酮（HTF）和柠檬黄酸（LA）是从柑桔（Citrus aurantium L.）花中分离得到的两个化合物。变种阿马拉具有多种生物活性。本研究以人肝细胞株HL-7702为研究对象，通过测定细胞毒性、细胞活力、肝标志酶、丙二醛（MDA）水平，探讨它们对四氯化碳（CCl 4）诱导的肝细胞损伤的保护作用。结果表明，HTF、LA预处理能显著逆转CCl 4诱导的HL-7702细胞活力下降，LA表现出较高的活性。HTF、LA均能降低CCl 4诱导的乳酸脱氢酶（LDH）、天门冬氨酸氨基转移酶（AST）漏出，抑制脂质过氧化，HTF的活性更强。由于HTF、LA无毒性，因此HTF、LA可有效地保护肝细胞免受CCl 4诱导的损伤。
5-Hydroxy-6,7,3′,4′-tetramethoxyflavone (HTF), limonexic acid (LA) are two compounds isolated from the flowers ofCitrus aurantiumL. var. amara Engl with various biological activities. This study was designed to investigate their protective effects against carbon tetrachloride (CCl4)-induced hepatocyte injury, using human hepatic cell line HL-7702 to determine the cell cytotoxicity, cell viability, levels of hepatic marker enzymes, malondialdehyde (MDA). Results showed that pretreatment with HTF, LA could significantly reverse CCl4-induced HL-7702 cell viability decrease, LA displayed a higher activity. HTF, LA also showed their capability of decreasing the CCl4-induced leakage of lactate dehydrogenase (LDH), aspartate aminotransferase (AST), inhibiting the lipid peroxidation, HTF showed more significant activity. Given that HTF, LA were not toxic, it is concluded that HTF, LA could effectively protect hepatocyte against CCl4-induced injury.