Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus
Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus
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DOI：
10.1128/jcm.41.10.4521-4524.2003
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发表时间：
2003-10-01
影响因子：
9.4
通讯作者：
Peiris, JSM
中科院分区：
文献类型：
--
作者：
Yam, WC;Chan, KH;Peiris, JSM
对两个世界卫生组织（WHO）严重急性呼吸系统综合征（SARS）网络实验室（WHO SARS网络实验室，分别位于香港大学[WHO-HKU]和德国汉堡Bernhard-Nocht研究所[WHO-Hamburg]）的逆转录（RT）-PCR方法进行了评价，以快速诊断与香港SARS相关的新型冠状病毒（CoV）。从163例疑似SARS患者中采集临床标本303份。WHO-HKU和WHO-Hamburg RT-PCR检测的终点均确定为0.1 50%组织培养感染剂量。以冠状病毒血清转化为SARS冠状病毒诊断的“金标准”，WHO-香港大学和WHO-汉堡RT-PCR检测的诊断灵敏度分别为61%和68%（鼻咽抽吸物样本）、65%和72%（咽拭子样本）、50%和54%（尿液样本）以及58%和63%（粪便样本），总体特异性为100%。对于确认患有SARS CoV并采集了两份或更多呼吸道标本的患者，检测第二份标本将WHO-香港大学和WHO-汉堡RT-PCR检测的灵敏度分别从64%和71%提高到75%和79%。检测一个以上的呼吸道标本将最大限度地提高PCR检测SARS CoV的灵敏度。
The reverse transcription (RT)-PCR protocols of two World Health Organization (WHO) severe acute respiratory syndrome (SARS) network laboratories (WHO SARS network laboratories at The University of Hong Kong [WHO-HKU] and at the Bernhard-Nocht Institute in Hamburg, Germany [WHO-Hamburg]) were evaluated for rapid diagnosis of a novel coronavirus (CoV) associated with SARS in Hong Kong. A total of 303 clinical specimens were collected from 163 patients suspected to have SARS. The end point of both WHO-HKU and WHO-Hamburg RT-PCR assays was determined to be 0.1 50% tissue culture infective dose. Using seroconversion to CoV as the "gold standard" for SARS CoV diagnosis, WHO-HKU and WHO-Hamburg RT-PCR assays exhibited diagnostic sensitivities of 61 and 68% (nasopharyngeal aspirate specimens), 65 and 72% (throat swab specimens), 50 and 54% (urine specimens), and 58 and 63% (stool specimens), respectively, with an overall specificity of 100%. For patients confirmed to have SARS CoV and from whom two or more respiratory specimens were collected, testing the second specimen increased the sensitivity from 64 and 71% to 75 and 79% for the WHO-HKU and WHO-Hamburg RT-PCR assays, respectively. Testing more than one respiratory specimen will maximize the sensitivity of PCR assays for SARS CoV.