The Biomechanical Effect of Corneal Collagen Cross-Linking (CXL) With Riboflavin and UV-A is Oxygen Dependent
The Biomechanical Effect of Corneal Collagen Cross-Linking (CXL) With Riboflavin and UV-A is Oxygen Dependent
复制标题
DOI：
10.1167/tvst.2.7.6
复制
发表时间：
2013-11-01
影响因子：
3
通讯作者：
Hafezi, Farhad
中科院分区：
文献类型：
--
作者：
Richoz, Olivier;Hammer, Arthur;Hafezi, Farhad
目的：为了减少核黄素和紫外线- a （UV-A）在角膜胶原交联（CXL）中的治疗时间，最近的方案修改包括在高通量下缩短照射时间，同时保持恒定的总施加能量（bunsenroscoe互易定律）。虽然这种参数变化可能在一定范围内产生类似的生物学结果，但互惠的极限是未知的。角膜氧扩散能力的局限性及其对CXL疗效的潜在影响，引起了人们对高通量CXL和经上皮CXL效率的关注。方法：用9 mW/cm(2)的CXL在两种不同的气氛下处理猪角膜：一种是常规氧含量（21%），另一种是氦气补充的低氧环境(
Purpose: In an attempt to reduce treatment time in corneal collagen cross-linking (CXL) with riboflavin and ultraviolet-A (UV-A), recent protocol modifications include shorter irradiation times at higher fluence, while maintaining constant total applied energy (Bunsen-Roscoe law of reciprocity). While such parameter changes might produce similar biological results within a certain range, the limits of reciprocity are unknown. Limitations in the corneal oxygen diffusion capacity and its potential impact on the efficacy of CXL, raise concerns regarding the efficiency of high-fluence CXL, and also of transepithelial CXL.Methods: Porcine corneas were treated with an epithelium-off CXL at a fluence of 9 mW/cm(2) under two different atmospheres: one with a regular oxygen content (21%) and another in a helium-supplemented, low-oxygen environment (