PYRROLINE-5-CARBOXYLATE IN HUMAN-PLASMA
PYRROLINE-5-CARBOXYLATE IN HUMAN-PLASMA
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DOI：
10.1016/0026-0495(84)90215-4
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发表时间：
1984-01-01
影响因子：
9.8
通讯作者：
PHANG, JM
中科院分区：
文献类型：
--
作者：
FLEMING, GA;HAGEDORN, CH;PHANG, JM
吡咯啉-5-羧酸是脯氨酸、鸟氨酸和谷氨酸相互转化的中间体。最近的研究表明，吡咯啉-5-羧酸在广泛的组织中具有调节特性。人体生理学研究受到该化合物缺乏足够灵敏的检测方法的限制。到目前为止，在正常人的血浆中或甚至在II型高脯氨酸血症患者的血浆中尚未检测到吡咯啉-5-羧酸。现在报道了一种通过制备纯化的大肠杆菌吡咯啉-5-羧酸还原酶来测量吡咯啉-5-羧酸的方法。该方法比目前可用的方法灵敏2或3个对数，适用于所有生物液体。在正常血浆、尿液和唾液中定量测定吡咯啉-5-羧酸盐。此外，吡咯啉-5-羧酸酯水平为10 × 10 - 10。至20倍。2例Ⅱ型高脯氨酸血症患者的血清蛋白正常。测量吡咯啉-5-羧酸盐的生理浓度的能力现在能够进一步表征其作为调节分子的作用。
Pyrroline-5-carboxylate is the intermediate in the interconversions of proline, ornithine and glutamate. Recent studies have suggested that pyrroline-5-carboxylate has regulatory properties in a wide range of tissues. Physiologic studies in humans have been limited by the unavailability of a sufficiently sensitive assay for this compound. Until now pyrroline-5-carboxylate has not been detectable in plasma of normal humans or even in plasma of patients with type II hyperprolinemia. A method is now reported for measuring pyrroline-5-carboxylate made possible by a preparation of purified Escherichia coli pyrroline-5-carboxylate reductase. This method is more sensitive than currently available methods by 2 or 3 logs, and is applicable for all biologic fluids. Pyrroline-5-carboxylate was quantitated in normal plasma, urine and saliva. In addition, pyrroline-5-carboxylate levels 10 .times. to 20 .times. normal were found in 2 patients with type II hyperprolinemia. The ability to measure physiologic concentrations of pyrroline-5-carboxylate now enables further characterization of its role as a regulatory molecule.