Patterned Polyvinyl Alcohol Hydrogel Dressings with Stem Cells Seeded for Wound Healing
Patterned Polyvinyl Alcohol Hydrogel Dressings with Stem Cells Seeded for Wound Healing
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带有干细胞种子的图案化聚乙烯醇水凝胶敷料用于伤口愈合
DOI：
10.3390/polym11010171
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发表时间：
2019-01-01
期刊：
影响因子：
5
通讯作者：
Chen, Jie
中科院分区：
文献类型：
--
作者：
Gao, Tianlin;Jiang, Menghui;Chen, Jie
聚乙烯醇（PVA）水凝胶和干细胞治疗已广泛应用于伤口愈合。但PVA缺乏生物活性和干细胞治疗的安全性限制了其应用。在这项研究中，脂肪干细胞（ADSCs）接种PVA敷料（ADSCs/PVA）的伤口愈合。PVA敷料的一侧通过紫外线（UV）照射（Az-Gel@PVA）用光反应明胶（Az-Gel）修饰，因此ADSCs可以在PVA敷料上粘附、增殖，并保持敷料的另一侧而不粘附于伤口。通过扫描电镜（SEM）和材料性能测试仪对Az-Gel@PVA的结构和力学性能进行了表征。通过细胞计数和活-死染色观察ADSCs的粘附和增殖情况。最后，利用体外和体内实验来证实ADSCs/PVA敷料对伤口愈合的作用。结果表明，Az-Gel被固定在PVA上，对PVA水凝胶的力学性能影响不大。表面修饰的PVA能促进ADSCs的粘附和增殖。蛋白释放试验表明，脂肪干细胞分泌的生物活性因子可渗透到创面。最后，体外和体内实验均表明ADSCs/PVA可通过分泌ADSCs的生物活性因子促进创面愈合。推测ADSC/PVA敷料不仅可以促进伤口愈合，还为干细胞的安全应用提供了一种新的途径，在皮肤组织工程中具有巨大的潜力。
Polyvinyl alcohol (PVA) hydrogel and stem cell therapy have been widely used in wound healing. However, the lack of bioactivity for PVA and security of stem therapy limited their application. In this study, an adipose-derived stem cells (ADSCs)-seeded PVA dressing (ADSCs/PVA) was prepared for wound healing. One side of the PVA dressing was modified with photo-reactive gelatin (Az-Gel) via ultraviolet (UV) irradiation (Az-Gel@PVA), and thus ADSCs could adhere, proliferate on the PVA dressings and keep the other side of the dressings without adhering to the wound. The structure and mechanics of Az-Gel@PVA were determined by scanning electron microscopy (SEM) and material testing instruments. Then, the adhesion and proliferation of ADSCs were observed via cell counts and live-dead staining. Finally, in vitro and in vivo experiments were utilized to confirm the effect of ADSCs/PVA dressing for wound healing. The results showed that Az-Gel was immobilized on the PVA and showed little effect on the mechanical properties of PVA hydrogels. The surface-modified PVA could facilitate ADSCs adhesion and proliferation. Protein released tests indicated that the bioactive factors secreted from ADSCs could penetrated to the wound. Finally, in vitro and in vivo experiments both suggested the ADSCs/PVA could promote the wound healing via secreting bioactive factors from ADSCs. It was speculated that the ADSCs/PVA dressing could not only promote the wound healing, but also provide a new way for the safe application of stem cells, which would be of great potential for skin tissue engineering.