Complete genomic sequence of bacteriophage J2-1: A novel Pseudoalteromonas phenolica phage isolated from the coastal water of Qingdao, China

Complete genomic sequence of bacteriophage J2-1: A novel Pseudoalteromonas phenolica phage isolated from the coastal water of Qingdao, China
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噬菌体 J2-1 的完整基因组序列:从中国青岛沿海水域分离的新型酚类假交替单胞菌噬菌体

DOI:
10.1016/j.margen.2017.12.001
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发表时间:
2018-06-01
期刊:
影响因子:
1.9
通讯作者:
McMinn, Andrew
McMinn, Andrew
中科院分区:
生物学4区
文献类型:
--
作者:
Liu, Qian;Han, Yuye;McMinn, Andrew

文献摘要

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噬菌体J2-1于2015年7月从中国青岛附近的表层沿海海水中分离。这是首次分离到抗苯酚假交替单胞菌CECT 7645(T)的噬菌体。使用透射电子显微镜的形态学分析显示,噬菌体J2-1具有87 +/-3 nm的头部,111 +/-3 nm的长尾,并且似乎属于肌尾病毒科。J2-1的基因组分析结果表明,基因组大小为142,204 bp,G + C含量为37.92%,有180个开放阅读框。总体上,ORF序列占基因组序列的89%。使用NCBI数据库中的BLACKS工具进行的基因组比较发现,J2-1与其他已知的突变体之间没有明显的相似性。
Phage J2-1 was isolated from surface coastal seawater off Qingdao, China in July 2015. It is the first time that a phage active against Pseudoalteromonas phenolica CECT 7645(T) has been isolated. Morphological analysis using transmission electron microscopy revealed that phage J2-1 has an 87 +/- 3 nm head, a 111 +/- 3 nm long tail and appears to belong to the family Myoviridae. Genomic analysis of J2-1 revealed a genome size of 142,204 bp with 37.92% G + C content and 180 ORFs. Overall the ORF sequences accounted for 89% of the genome sequence. A genome comparison using the BLASTN tool in the NCBI database found no obvious similarity between J2-1 and other known phages.