DISTRIBUTION AND SYNTHESIS OF HISTONE-1 SUBFRACTIONS DURING SPERMATOGENESIS IN THE RAT
DISTRIBUTION AND SYNTHESIS OF HISTONE-1 SUBFRACTIONS DURING SPERMATOGENESIS IN THE RAT
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DOI：
10.1016/0014-4827(82)90162-8
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发表时间：
1982-01-01
影响因子：
3.7
通讯作者：
MEISTRICH, ML
中科院分区：
文献类型：
--
作者：
BUCCI, LR;BROCK, WA;MEISTRICH, ML
研究了不同组蛋白1 （H1）亚组分在纯化大鼠睾丸生发细胞中的分布和合成。采用SDS（十二烷基硫酸钠）聚丙烯酰胺凝胶电泳法将大鼠睾丸H1分离成4个条带，其中包含H1亚段H1b+H1d+H1e （H1bde）、H1a、H1c和H1t。结果表明，在精原细胞和早期初级精母细胞中存在并合成了体细胞H1亚片段H1a、b、c、d和e。H1a，一个次要的体细胞亚分支，是这些细胞中主要的H1亚分支（占总H1的45%）。睾丸特异性H1亚段。在这些细胞中几乎检测不到H1t。然而，在粗粒精母细胞中，H1t（占总H1的56%）取代了H1b， c， d和e，并且H1a的水平仍然很高（占总H1的30%）。H1t是粗线精母细胞中唯一活跃合成的亚组分。早期精子细胞（步骤1-8）具有与粗线期精子细胞相同的亚组分组成，但H1合成极低。H1亚型的基因表达受发育控制。似乎与DNA复制相关的H1a亚组分的合成升高。H1t合成定位于粗线精母细胞，对应于H2B和H2A睾丸特异性变异的调控。
The distribution and synthesis of various histone 1 (H1) subfractions were studied in purified populations of rat testis germinal cells. Rat testis H1 was resolved by SDS [sodium dodecyl sulfate] polyacrylamide gel electrophoresis into 4 bands containing H1 subfractions H1b+H1d+H1e (H1bde), H1a, H1c and H1t. Results show that the somatic H1 subfractions H1a, b, c, d and e are present and synthesized in spermatogonia and early primary spermatocytes. H1a, a minor somatic subfraction, is the predominant H1 subfraction in these cells (45% of total H1). The testis-specific H1 subfraction. H1t, is barely detectable in these cells. In pachytene spermatocytes, however, H1t (56% of total H1) has replaced H1b, c, d and e, and levels of H1a remain high (30% of total H1). H1t is the only subfraction actively synthesized in pachytene spermatocytes. Early spermatids (steps 1-8) have the same subfraction composition as pachytene spermatocytes, but extremely low H1 synthesis occurs. Gene expression of H1 subfractions is under developmental control. There appears to be an elevated synthesis of subfraction H1a associated with DNA replication. The localization of H1t synthesis to the pachytene spermatocytes corresponds to the regulation of testis-specific variants of H2B and H2A.