RAPID ACTIVATION OF ALTERNATIVE 2ND MESSENGER PATHWAYS IN OLFACTORY CILIA FROM RATS BY DIFFERENT ODORANTS
RAPID ACTIVATION OF ALTERNATIVE 2ND MESSENGER PATHWAYS IN OLFACTORY CILIA FROM RATS BY DIFFERENT ODORANTS
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DOI：
10.1002/j.1460-2075.1990.tb07422.x
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发表时间：
1990-08-01
期刊：
影响因子：
11.4
通讯作者：
BREER, H
中科院分区：
文献类型：
--
作者：
BOEKHOFF, I;TAREILUS, E;BREER, H
嗅觉感受细胞中化学电信号传导的分子机制至今仍不清楚。在这项研究中，气味诱导的第二信使在大鼠嗅纤毛的形成监测在亚秒的时间范围内使用快速动力学装置。微摩尔浓度的citralva的应用诱导环磷酸腺苷水平的快速，短暂的升高，而肌醇三磷酸的浓度不受影响。与此相反，吡嗪引起三磷酸肌醇浓度的上升，不影响环磷酸腺苷的水平。对加臭剂诱导反应的动力学参数分析表明，两个体系显然是同时进行的。气味剂的激活作用似乎是通过不同的G蛋白介导的。因此，至少有两种不同的第二信使途径似乎参与嗅觉信号转导。
The molecular mechanisms mediating the chemoelectrical signal transduction in olfactory receptor cells are still elusive. In this study odor induced formation of second messengers in rat olfactory cilia was monitored in a subsecond time range using a rapid kinetic device. Application of micromolar concentration of citralva induced a rapid, transient elevation of the cyclic adenosine monophosphate level, whereas the concentration of inositol triphosphate was not affected. In contrast, pyrazine caused a rise in the concentration of inositol trisphosphate, not affecting the level of cyclic adenosine monophosphate. Analysis of the kinetic parameter for the odorant induced reaction indicated that apparently two systems are operating simultaneously. The activating effects of odorants appear to be mediated via different G-proteins. Thus, at least two different second messenger pathways appear to be involved in olfactory signal transduction.