Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress
Inhalation of hydrogen gas suppresses hepatic injury caused by ischemia/reperfusion through reducing oxidative stress
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DOI：
10.1016/j.bbrc.2007.07.088
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发表时间：
2007-09-28
影响因子：
3.1
通讯作者：
Ohta, Shigeo
中科院分区：
文献类型：
--
作者：
Fukuda, Kei-Ichi;Asoh, Sadamitsu;Ohta, Shigeo
我们最近发现，分子氢具有选择性减少细胞毒性活性氧（如羟基自由基）的巨大潜力，吸入氢气可通过减少氧化应激减少脑梗死体积[I]。Ohsawa，M.石川，K.高桥，M.渡边K. Nishimaki，K. Yamagata，K.- I.桂属，澳-地片山南Asoh，S. Ohta，Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals，Nat. Med.，13（2007）688-694]。在这里，我们表明，吸入氢气是适用于缺血/再灌注引起的肝损伤，使用小鼠。门三联体的左叶和左中叶的肝脏完全闭塞90分钟，然后再灌注180分钟。吸入氢气（1-4%）在最后190分钟抑制肝细胞死亡，血清丙氨酸氨基转移酶和肝丙二醛的水平降低。相比之下，氦气没有显示出保护作用，这表明氢气的保护作用是特异性的。因此，我们建议吸入氢气是一种广泛适用的方法，以减少氧化应激。(c)2007年爱思唯尔公司All rights reserved.
We have recently showed that molecular hydrogen has great potential for selectively reducing cytotoxic reactive oxygen species, such as hydroxyl radicals, and that inhalation of hydrogen gas decreases cerebral infarction volume by reducing oxidative stress [I. Ohsawa, M. Ishikawa, K. Takahashi, M. Watanabe, K. Nishimaki, K. Yamagata, K.-I. Katsura, Y. Katayama, S. Asoh, S. Ohta, Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals, Nat. Med., 13 (2007) 688-694]. Here we show that the inhalation of hydrogen gas is applicable for hepatic injury caused by ischemia/reperfusion, using mice. The portal triad to the left lobe and the left middle lobe of the liver were completely occluded for 90 min, followed by reperfusion for 180 min. Inhalation of hydrogen gas (1-4%) during the last 190 min suppressed hepatic cell death, and reduced levels of serum alanine aminotransferase and hepatic malondialdehyde. In contrast, helium gas showed no protective effect, suggesting that the protective effect by hydrogen gas is specific. Thus, we propose that inhalation of hydrogen gas is a widely applicable method to reduce oxidative stress. (c) 2007 Elsevier Inc. All rights reserved.