OXA-58, a novel class D β-lactamase involved in resistance to carbapenems in Acinetobacter baumannii
OXA-58, a novel class D β-lactamase involved in resistance to carbapenems in Acinetobacter baumannii
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DOI：
10.1128/aac.49.1.202-208.2005
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发表时间：
2005-01-01
影响因子：
4.9
通讯作者：
Nordmann, P
中科院分区：
文献类型：
--
作者：
Poirel, L;Marqué, S;Nordmann, P
2003年在法国图卢兹分离出碳青霉烯类耐药鲍曼不动杆菌菌株。在大肠杆菌中的克隆和表达鉴定了碳青霉烯水解β-内酰胺酶OXA-58，其与其他苯唑西林酶弱相关（小于50%氨基酸同一性）。它水解青霉素、苯唑西林和亚胺培南，但不水解广谱头孢菌素。bla（OXA-58）基因定位于一株CA. 30-kb非自身转移质粒。在A.鲍曼不动杆菌CIP 7010（T）参考菌株，其赋予对碳青霉烯类的降低的敏感性。bla（OXA-58）基因被两个新的ISAba 3样插入元件包围。本研究描述了一种新发现的β-内酰胺酶，它可能导致A.鲍曼不动杆菌。
A carbapenem-resistant Acinetobacter baumannii strain was isolated in Toulouse, France, in 2003. Cloning and expression in Escherichia coli identified the carbapenem-hydrolyzing beta-lactamase OXA-58, which is weakly related (less than 50% amino acid identity) to other oxacillinases. It hydrolyzed penicillins, oxacillin, and imipenem but not expanded-spectrum cephalosporins. The bla(OXA-58) gene was located on a ca. 30-kb non-self-transferable plasmid. After electrotransformation in the A. baumannii CIP7010(T) reference strain, it conferred reduced susceptibility to carbapenems. The bla(OXA-58) gene was bracketed by two novel ISAba3-like insertion elements. This study describes a newly characterized P-lactamase that may contribute to carbapenem resistance in A. baumannii.