Conserved guanine-guanine stacking in tetraplex and duplex DNA
Conserved guanine-guanine stacking in tetraplex and duplex DNA
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DOI：
10.1007/s002490100174
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发表时间：
2001-12-01
影响因子：
2
通讯作者：
Vorlícková, M
中科院分区：
文献类型：
--
作者：
Kypr, J;Fialová, M;Vorlícková, M
使用一系列适当选择的寡核苷酸，我们证明了d（CCCCGGGG）的DNA双链体提供了与d（GGGG）的平行链四链体几乎相同的CD谱。CD光谱对DNA中的碱基堆积非常敏感，因此上述观察结果表明，在d（CCCCGGGG）的双链体和d（GGGG）的四链体中，鸟嘌呤-鸟嘌呤堆积基本相同。由三氟乙醇诱导的d（CCCCGGGG）的A型也提供了非常相似的CD光谱。这些结果表明，鸟嘌呤-鸟嘌呤堆积是一个结构不变的保守在各种核酸构象。结构不变性可能与遗传分子的进化相一致，并且对于基本功能（例如转录的起始）是重要的。
Using a series of suitably chosen oligonucleotides, we demonstrate that the DNA duplex of d(CCCCGGGG) provides an almost identical CD spectrum as the parallel -stranded tetraplex of d(GGGG). The CD spectra are very sensitive to base stacking in DNA so that the above observation indicates that guanine-guanine stacking is essentially the same within the duplex of d(CCCCGGGG) and the tetraplex of d(GGGG). A very similar CD spectrum is also provided by the A-form of d(CCCCGGGG) induced by trifluoroethanol. These results reveal that guanine-guanine stacking is a structural invariant conserved in various nucleic acid conformers. The structural invariance is likely to cohere with evolution of the genetic molecules and be important for fundamental functions, e.g. initiation of transcription.