Regulation of mouse soluble interleukin-6 receptor secretion by decidua.
Regulation of mouse soluble interleukin-6 receptor secretion by decidua.
复制标题
蜕膜对小鼠可溶性白细胞介素 6 受体分泌的调节。
DOI：
10.1006/bbrc.1995.1921
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
A. Miyake
中科院分区：
文献类型：
--
作者：
M. Yamaguchi;A. Mammoto;T. Taga;T. Kishimoto;A. Miyake
使用小鼠蜕膜的原代细胞培养物检测mIL-6和8-溴cAMP对msIL-6 R分泌的调节。用胶原酶消化妊娠第11天的小鼠蜕膜，并将蜕膜细胞培养长达3天。加入mIL-6和8-溴cAMP导致在培养的第2天显著抑制培养的小鼠蜕膜细胞的msIL-6 R分泌。这种作用是剂量依赖性的，引起msIL-6 R分泌显著抑制的mIL-6和8-溴cAMP的最低浓度分别为250 pM和50 μ M。北方印迹分析表明，用8-溴cAMP处理蜕膜细胞显著降低mIL-6 R mRNA的稳态水平。然而，用mIL-6处理蜕膜细胞并不影响mIL-6 R mRNA的稳态水平。这些结果表明，mIL-6降低msIL-6 R的分泌，而不改变mIL-6 R mRNA的稳态水平，和cAMP是在小鼠蜕膜细胞中参与这种下调msIL-6 R分泌的第二信使之一。
Regulation of msIL-6R secretion by mIL-6 and 8-bromo cAMP was examined using primarily cell culture of mouse decidua. Mouse decidua on day 11 of pregnancy was digested by collagenase and decidual cells were cultured for up to 3 days. Addition of mIL-6 and 8-bromo cAMP resulted in significant inhibition of msIL-6R secretion from cultured mouse decidual cells by the 2nd day in culture. The effects were dose-dependent and the lowest concentrations of mIL-6 and 8-bromo cAMP which cause significant inhibition of msIL-6R secretion were 250 pM and 50 microM, respectively. Northern blot analysis indicated that treatment of decidual cells with 8-bromo cAMP significantly decreased the steady-state level of mIL-6R mRNA. However, treatment of decidual cells with mIL-6 did not affect the steady-state level of mIL-6R mRNA. These results suggest that mIL-6 decreases msIL-6R secretion without changing of the steady-state level of mIL-6R mRNA, and that cAMP is one of the second messengers in mouse decidual cells involved in this down regulation of msIL-6R secretion.