Monoclonal leukocyte antibody does not decrease the injury of transient focal cerebral ischemia in cats.
Monoclonal leukocyte antibody does not decrease the injury of transient focal cerebral ischemia in cats.
复制标题
单克隆白细胞抗体不能减轻猫短暂性局灶性脑缺血的损伤。
DOI：
10.1161/01.str.23.2.247
复制
发表时间：
1992
期刊：
影响因子：
8.3
通讯作者：
Traystman,RJ
中科院分区：
文献类型：
--
作者：
Takeshima,R;Kirsch,JR;Koehler,RC;Gomoll,AW;Traystman,RJ
我们测试的假设，即抑制白细胞功能的单克隆抗体60.3（MoAb 60.3）的管理，提高电生理恢复和减少损伤体积后短暂局灶性脑缺血猫。氟烷麻醉猫进行了90分钟的左侧大脑中动脉和双侧颈总动脉闭塞，然后再灌注180分钟。猫在缺血45分钟时以盲法接受2 mg/kg针对CDw 18白细胞抗原复合物的MoAb 60.3（n = 8）或等体积的稀释剂（无菌盐水; n = 10）。缺血后充血发生在左侧尾状核，而其他脑区的血流恢复到控制。没有区域表现出延迟性低灌注，组间无差异。在缺血期间消融记录在左侧皮层的体感诱发电位，并在两组中再灌注180分钟时恢复到基线幅度的10%以下。通过氯化2，3，5-三苯基四唑染色评估的左半球损伤体积不受药物治疗的影响（平均+/- SE值：MoAb 60.3，37 +/- 5%;安慰剂，半球的38 +/- 7%）。
We tested the hypothesis that inhibition of leukocyte function by administration of monoclonal antibody 60.3 (MoAb 60.3) improves electrophysiological recovery and decreases injury volume following transient focal cerebral ischemia in cats.Halothane-anesthetized cats underwent 90 minutes of left middle cerebral artery and bilateral common carotid artery occlusion followed by 180 minutes of reperfusion. Cats were assigned to receive either 2 mg/kg MoAb 60.3 (n = 8) directed at the CDw18 leukocyte antigen complex or an equal volume of diluent (sterile saline; n = 10) at 45 minutes of ischemia in a blinded fashion.Blood flow to the left temporoparietal cortex decreased to less than 5 ml/min/100 g with ischemia, but was minimally affected on the right side. Postischemic hyperemia occurred in the left caudate nucleus, whereas blood flow in other brain regions returned to control. No region demonstrated delayed hypoperfusion, and there were no differences between groups. Somatosensory evoked potential recorded over the left cortex was ablated during ischemia and recovered to less than 10% of baseline amplitude at 180 minutes of reperfusion in both groups. Left hemispheric injury volume, as assessed by 2,3,5-triphenyltetrazolium chloride staining, was not affected by drug treatment (mean +/- SE values: MoAb 60.3, 37 +/- 5%; placebo, 38 +/- 7% of hemisphere).Inhibition of leukocyte function with MoAb 60.3 does not afford protection from severe focal ischemia and reperfusion in cats.